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Optimization of a multi‐stage,multi‐subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites
Authors:Holger Spiegel  Helga Schinkel  Robin Kastilan  Pia Dahm  Alexander Boes  Matthias Scheuermayer  Ivana Chudobová  Dominika Maskus  Rolf Fendel  Stefan Schillberg  Andreas Reimann  Rainer Fischer
Institution:1. +49‐241‐6085‐12461+49‐241‐6085‐10000;2. Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany;3. Research Center for Infectious Diseases, University of Würzburg, Würzburg, Germany;4. Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany
Abstract:
Keywords:Pichia pastoris  Plasmodium falciparum  proteolytic degradation  protein engineering
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