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Carboxypeptidase D is the only enzyme responsible for antibody C‐terminal lysine cleavage in Chinese hamster ovary (CHO) cells |
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| Authors: | Zhilan Hu Henry Zhang Benjamin Haley Frank Macchi Feng Yang Shahram Misaghi Joseph Elich Renee Yang Yun Tang John C Joly Bradley R Snedecor Amy Shen |
| Institution: | 1. Department of Early Stage Cell Culture, Genentech Inc., 1 DNA Way, South San Francisco, California 94080;2. Department of Molecular Biology, Genentech Inc., 1 DNA Way, South San Francisco, California;3. Department of Analytical Development and Quality Control, Genentech Inc., 1 DNA Way, South San Francisco, California;4. (650) 225‐6446(650) 225‐2006 |
| Abstract: | |
| Keywords: | CHO cell charge variants C‐terminal lysine carboxypeptidase D (CpD) RNAi (RNA interference) shRNA (small interference RNA) CRISPR (clustered regularly interspaced short palindromic repeats) knockout |