Flow‐injection chemiluminescence determination of olanzapine using N‐chlorosuccinimide–calcein reaction sensitized by zinc (II)

A novel, rapid and sensitive chemiluminescence (CL) method combined with flow‐injection (FI) has been established for the estimation of olanzapine. This method is based on the CL signal generated between N‐chlorosuccinimide and olanzapine in an alkaline medium in the presence of calcein and Zn(II). Under optimum conditions, the CL signal was proportional to the olanzapine concentration ranging from 1.0 × 10^‐10 to 3.0 × 10^‐7 g/mL. The detection limit is 8.9 × 10^‐11 g/mL olanzapine (3σ) and the relative standard deviation for 3.0 × 10^‐9 g/mL of olanzapine is 1.9% (n = 11). The current CL method was applied to determine olanzapine in pharmaceutical formulations and biological fluids with satisfactory results. The possible CL reaction mechanism is discussed briefly. Copyright © 2013 John Wiley & Sons, Ltd.