Abstract: | A dual‐function fluorescent probe (Probe 1 ) was developed for discriminative detection of Hg2+ and N2H4. Probe 1 could discriminatively detect Hg2+ and N2H4 through two different reaction sites, with the mechanism for Probe 1 for Hg2+ depending on a desulfurization reaction and for N2H4 depending on the Schiff‐base reaction. N2H4 had minimal effect on Hg2+ detection in dimethyl sulfoxide (DMSO)/H2O solution, but Hg2+ could interfere with N2H4 detection in DMSO/buffer solution. Different concentrations of Hg2+ and N2H4 resulted in different blue shades of Probe 1 test strips, and the shade of blue was different with the same concentration of Hg2+ or N2H4, as observed under ultraviolet light at 365 nm wavelength. |