Resonance light‐scattering enhancement effect of the protein–Y3+–TTA–SLS system and its analytical application |
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| Authors: | Changying Guo Xia Wu Wei Xu Jinghe Yang |
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| Institution: | 1. Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, People's Republic of China;2. Central Laboratory, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, People's Republic of China |
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| Abstract: | In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris–HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y3+–2‐thenoyltrifluoroacetone (TTA)–sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 × 10?9–1.0 × 10?5 g/mL for BSA, 1.0 × 10–8–1.0 × 10?5 g/mL for HSA and 1.0 × 10–8–1.0 × 10?6 g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied. Copyright © 2008 John Wiley & Sons, Ltd. |
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| Keywords: | resonance light scattering determination protein Y3+– TTA– SLS |
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