Terbium‐sensitized fluorescence method for the determination of deferasirox in biological fluids and tablet formulation |
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| Authors: | Jamshid L Manzoori Abolghasem Jouyban Mohammad Amjadi Vahid Panahi‐Azar Elnaz Tamizi Jalil Vaez‐Gharamaleki |
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| Institution: | 1. Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran;2. Department of Pharmaceutical and Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;3. Pharmaceutical Analysis Lab., Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Hematology‐Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
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| Abstract: | A novel, rapid and sensitive spectroflurimetric method was developed and validated for the determination of deferasirox in urine, serum and tablet samples based on sensitization of terbium fluorescence. The excitation and emission wavelengths were 328 and 545 nm, respectively. The optimum conditions for the determination of deferasirox were investigated considering the effects of various parameters. The method was quantitatively evaluated in terms of linearity, recovery, reproducibility and limit of detection. Under the optimal conditions, the fluorescence intensities were linear with the concentration of deferasirox in the range of 5 × 10?9 to 5×10?6 mol L?1, with a detection limit of 1.5 × 10?9 mol L?1 and a relative standard deviation of 1.1–2.3%. Linearity, reproducibility, recovery and limit of detection made the method suitable for determination of deferasirox in urine, serum and tablets samples. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | deferasirox fluorescence terbium‐sensitized luminescence urine serum |
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