Determination of puerarin in biological samples and its application to a pharmacokinetic study by flow‐injection chemiluminescence |
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Authors: | Ran Yang Qingwen Wang Huajin Zeng Zao Qin Jianjun Li Lingbo Qu |
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Institution: | 1. Department of Chemistry, Zhengzhou University, , Zhengzhou, 450001 People's Republic of China;2. School of Pharmaceutical Sciences, Zhengzhou University, , Zhengzhou, 450001 People's Republic of China;3. School of Chemistry and Chemical Engineering, Henan University of Technology, , Zhengzhou, 450001 People's Republic of China |
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Abstract: | A simple and sensitive flow injection–chemiluminescence (FI–CL) method has been developed for the determination of puerarin, based on the fact that puerarin can greatly inhibit CL of the luminol–H2O2–haemoglobin system. The inhibition of CL intensity was linear to the logarithm of the concentration of puerarin in the range 0.08–10.0 μg/mL (r2 = 0.9912). The limit of detection was 0.05 μg/mL (3σ) and the relative standard deviation (RSD) for 1.0 μg/mL (n = 11) of puerarin solution was 1.4%. Coupled with solid‐phase extraction (SPE) as the sample pretreatment, the determination of puerarin in biological samples and a preliminary pharmocokinetic study of puerarin in rats were performed. The recoveries for plasma and urine at three different concentrations were 89.2–110.0% and 91.4–104.8%, respectively. The pharmacokinetics of puerarin in plasma of rat coincides with the two‐compartment open model. The T1/2α, T1/2β, CL/F, VZ/F, AUC(0 – t), MRT(0 – ∞), Tmax and Cmax were 0.77 ± 0.21 h, 7.55 ± 2.64 h, 2.43 ± 1.02 L/kg/h, 11.40 ± 3.45 L/kg, 56.67 ± 10.65 mg/h/L, 5.04 ± 2.78 h, 1.00 ± 0.35 h and 19.70 ± 4.67 μg/mL, respectively. Copyright © 2011 John Wiley & Sons, Ltd. |
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Keywords: | puerarin flow injection– chemiluminescence (FI– CL) solid‐phase extraction (SPE) biological samples pharmacokinetics |
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