Effect of copper on pro- and antioxidative reactions in radish (Raphanus sativus L.) in vitro and in vivo |
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| Institution: | 1. Department of Botany and Plant Physiology, N.P. Ogarjov Mordovia State University, Bolshevistskaja Str. 68, 430005 Saransk, Russia;2. Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387 Kraków, Poland;1. Department of Materials Science, M. V. Lomonosov Moscow State University, 119991 Moscow, Russian Federation;2. Department of Biology, M. V. Lomonosov Moscow State University, 119991 Moscow, Russian Federation;3. Institute of Physical Materials Science, Siberian Branch of the Russian Academy of Sciences, 670047 Ulan-Ude, Russian Federation;4. Department of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, Russian Federation;5. N. S. Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation;1. Department of Biology, College of Life Science, Huzhou University, Huzhou, Zhejiang, China;2. Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA;3. Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California-Davis, Davis, CA, USA;4. Department of Extension, College of Food, Agriculture, and Environmental Sciences, The Ohio State University, Columbus, OH, USA;5. South Centers, The Ohio State University, Piketon, OH, USA |
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| Abstract: | The generation of superoxide radicals, lipid peroxidation (as measured by malone dialdehyde formation) and the activity of selected antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase) were assessed in radish (Raphanus sativus L.), in response to elevated concentrations of copper ions in the culture medium in vitro and in vivo. Experiments were performed on 7-day-old seedlings and 5-week-old calluses grown on media supplemented with CuSO4 in concentrations of 10, 100 and 1000 μМ. The exposure to elevated Cu concentrations in the medium significantly reduced both callogenesis and the proliferation of radish calluses in vitro. Cu treatment resulted in the increased generation of the superoxide radical (O2 −) in radish seedlings and calluses indicating the occurrence of oxidative stress in radish cells, whereas the level of lipid peroxidation (LPO) remained unchanged. Both in calluses and in radish seedlings in vivo, the relative level of oxidative stress was maximal at micromolar Cu concentrations and became attenuated with increasing Cu concentrations. Stronger oxidative stress occurred in the radish seedlings in vivo, compared with radish calluses in vitro. The observed lower sensitivity of calluses to Cu-induced oxidative stress and their ability to proliferate upon exposure to Cu concentrations of up to 1000 μМ demonstrate the potential of in vitro cell-selection to obtain metal-tolerant radish plant lines. |
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| Keywords: | Callus Copper Oxidative stress Antioxidant enzymes |
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