The effects of early life lead exposure on the expression of P2X7 receptor and synaptophysin in the hippocampus of mouse pups |
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| Institution: | 1. Department of Neurophysiology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore, India;2. Forensic and Medical Systems, Axxonet Systems Technologies Pvt. Ltd., Bangalore, India;3. Department of Molecular and Cell Biology, University of Leicester, Leicester, United Kingdom;4. Leicester Institute of Structural and Chemical Biology, University of Leicester, Leicester, United Kingdom;1. Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India;2. Department of Microbiology, PSG College of Arts and Science, Coimbatore 641 016, Tamil Nadu, India;1. Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands;2. Nutricia Research, Uppsalalaan 12, 3584 CT Utrecht, The Netherlands;3. Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands;4. Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The Netherlands;5. Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands |
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| Abstract: | The present study was undertaken to investigate the effects of maternal lead exposure on expression of P2X7 receptor and synaptophysin in the hippocampus of mice offspring. Lead exposure initiated from beginning of gestation to weaning. Lead acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups, respectively. On the 21st postnatal day, the Pb levels were also determined by graphite furnace atomic absorption spectrometry. The expression of P2X7 receptor and synaptophysin in hippocampus was examined by immunohistochemistry and Western blotting. The lead levels in blood and hippocampus of all lead exposure groups were significantly higher than that of the control group (P < 0.05). Compared with the control group, the expression of P2X7 receptor was increased in lead exposed groups (P < 0.05), but the expression of synaptophysin was decreased (P < 0.05). The high expression of P2X7 receptor and low expression of synaptophysin in the hippocampus of pups may contribute to the neurotoxicity associated with maternal Pb exposure. |
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| Keywords: | Lead Hippocampus P2X7 receptor Synaptophysin Neurotoxicity |
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