In vitro anticancer and antibacterial activates of the yttrium(III) complex and its nano-carriers toward DNA cleavage and biological interactions with DNA and BSA; An experimental and computational studie |
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| Institution: | 1. Nano Bioeletrochemistry Research Center, Bam University of Medical Sciences, Bam, Iran;2. Department of Chemistry, University of Isfahan, Isfahan, Iran;3. Department of Physical and Environmental Sciences, University of Toronto Scarborough 1265 Military Trail, Toronto, Ontario, M1C 1A4, Canada;1. Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil;2. Laboratório Fotonanobio, Departamento Acadêmico de Física, Universidade Tecnológica Federal do Paraná, CEP 80.230-901, Curitiba, PR, Brazil;3. Laboratório de Células Inflamatórias e Neoplásicas, Departamento de Biologia Celular, Universidade Federal do Paraná, CEP 81.531-980, Curitiba, PR, Brazil;1. Physics Department, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516, Caparica, Portugal;2. LIBPhys - Laboratory for Instrumentation, Biomedical Engineering and Radiation Physics, Portugal;3. Anatomy Department, NOVA Medical School, NOVA University Lisbon, 1169-056, Lisbon, Portugal;1. Center for Advanced Studies and Technology (C.A.S.T.), \"G. d''Annunzio\" University of Chieti-Pescara, Via Luigi Polacchi 11, Chieti, I-66100, Italy;2. Department of Physics, Università Degli Studi di Milano, Via Celoria 16, Milano, I-20133, Italy;3. LASA, Department of Physics, Università Degli Studi di Milano and INFN-Milano, Via F.lli Cervi 201, Segrate, MI, I-20090, Italy;4. Institute of Clinical Immunotherapy and Advanced Biological Treatments, Piazza Pierangeli 1, Pescara, Rectorate of Leonardo da Vinci Telematic University, Largo San Rocco 11 Torrevecchia Teatina, CH, Italy;5. Department of Medicine and Aging Sciences, \"G. d''Annunzio\" University of Chieti-Pescara, via Luigi Polacchi 11, Chieti, I-66100, Italy;1. Department of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, Clinicilor St, 400006, Cluj-Napoca, Romania;2. Departments of Pharmaceutical Technology and Biopharmaceutics, “Iuliu Hatieganu” University of Medicine and Pharmacy, 12 Ion Creanga St., 400606, Cluj-Napoca, Romania;3. National Institute for Research and Development of Isotopic and Molecular Technologies, Donath St, No. 67-103, RO-400293, Cluj-Napoca, Romania;4. Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Ha?ieganu University of Medicine and Pharmacy, 4 Pasteur St., 400349, Cluj-Napoca, Romania;5. Department of Morphopathology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 35 Clinicilor St, 400006, Cluj-Napoca, Romania;6. INCDO-INOE 2000, Research Institute for Analytical Instrumentation, ICIA, Romania;7. Department of Surgery, “Iuliu Hatieganu” University of Medicine and Pharmacy, Babes St, 400012, Cluj-Napoca, Romania;8. University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Calea Manastur St, 400372, Cluj-Napoca, Romania;1. Univ. Grenoble Alpes, CEA, IRIG, 38000, Grenoble, France;2. Univ. Grenoble Alpes, INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), and Environmental and System Biology (BEeSy), 38000, Grenoble, France |
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| Abstract: | ObjectivesIn this research, the biological properties of the yttrium (III) (Y) complex, with 2,9-dimethyl- 1,10-phenanthroline (Me2Phen) ligand, were examined for in vitro fish DNA (FS-DNA)/ bovine serum albumin (BSA) interactions, DNA-cleavage, anticancer and antibacterial activities.MethodsMulti-spectrophotometric techniques and computational calculations were used for the interaction studies of the BSA and FS-DNA with the Y-complex. Absorption and fluorescence spectroscopy methods were used to define thermodynamic parameters, the binding constants (Kb), and the probable binding mechanism. Also, the DFT (density functional theory) study and molecular docking calculation of the Y-complex were done. Besides, the nanocarriers of Y-complex (lipid nanoencapsulation (LNEP) and the starch nanoencapsulation (SNEP)), as active anticancer candidates, were prepared. Finally, DNA-cleavage, anticancer, and antibacterial activities of this complex were investigated.ResultsThe absorption and fluorescence measurements were exhibited that the Y-complex has a high binding affinity to FS-DNA and BSA through a static mechanism. The negative thermodynamic parameter values for both DNA/BSA binding were confirmed that the hydrogen bonds and van der Waals forces played an essential role in the spontaneous bonding procedure. The site marker competitive studies for BSA confirmed that the Y-complex bonds to the sub-domain IB of protein (site III) on BSA, which was entirely agreement by docking calculation. The complex has displayed efficient DNA cleavage, antifungal and antibacterial activities. The anticancer activity of the Y-complex and its starch/lipid nano-encapsulated was carried out in cancer cell lines, which exposed considerably high activity.ConclusionsThus, Y-complex can be transported professionally through BSA in the blood and bonds in the groove of DNA. Base on biological applications of the Y-complex, it can be concluded that this complex and its nanocarriers can suggest as novel anticancer and antibacterial candidates. |
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| Keywords: | Yttrium(III) complex DNA/BSA binding Antibacterial/antitumor activities Nano-encapsulated DNA cleavage |
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