Biosynthesis of 1,4-butanediol from erythritol using whole-cell catalysis |
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| Authors: | Lu Dai Cui Tai Yali Guo |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People’s Republic of China;2. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People’s Republic of China;3. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People’s Republic of China |
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| Abstract: | 1,4-Butanediol (BDO) biosynthesis from renewable resources is of increasing interest because of global energy and environmental problems. We have previously demonstrated the production of BDO from erythritol by whole-cell catalysis. Here, the effects of several variables on BDO production were investigated, including cell density, temperature, substrate concentration and pH. It was found that the maximum BDO production was obtained at cell density (OD600) of 30. Low temperature and weak alkaline environment were beneficial for the biotransformation. Regarding substrate concentration, 80?g/L of erythritol was found to be optimum for the bioconversion. Under the optimal conditions, the highest concentration of BDO reached 34.5?mg/L, resulting in 5.8-fold increment after optimization. These results will provide useful guidance for enhancing the bioconversion of erythritol to BDO. |
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| Keywords: | 1 4-Butanediol erythritol whole-cell biocatalysis |
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