Transplantation of Foetal Neural Stem Cells into the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration |
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Authors: | Maria Concetta Geloso Stefano Giannetti Carlo Cenciarelli Manuela Budoni Patrizia Casalbore Giulio Maira Fabrizio Michetti |
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Institution: | (1) Institute of Anatomy and Cell Biology, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Rome, Italy;(2) Institute of Neurosurgery, Università Cattolica del Sacro Cuore, Rome, Italy;(3) Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy |
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Abstract: | The present study investigates the survival and fate of neural stem cells/progenitor cells (NSC/NPCs) homografted into the
hippocampus of rats treated with trimethyltin (TMT), a potent neurotoxicant considered a useful tool to obtain a well characterized
model of neurodegeneration, to evaluate their possible role in the reparative mechanisms that accompany neurodegenerative
events. NSC/NPCs expressing eGFP by lentivirus-mediated infection were stereotaxically grafted into the hippocampus of TMT-treated
animals and controls. Two weeks after transplantation surviving NSC/NPCs were detectable in 60% of TMT-treated animals and
30% of controls, while 30 days after transplantation only 40% of TMT-treated animals showed surviving grafted cells, which
were undetectable in controls. At both times investigated, while grafted NSC/NPCs differentiated into neurons or astrocytes
could be observed in addition to undifferentiated NSC/NPCs, we did not find evidence of structural integration of grafted
cells into the main site of hippocampal lesion leading to appreciable repair.
Maria Concetta Geloso and Stefano Giannetti contributed equally to this work. |
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Keywords: | Trimethyltin Neurodegeneration Hippocampus Neural stem cells Transplantation |
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