Determination of half-reaction equilibrium in a ping-pong enzyme mechanism |
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| Authors: | Geoffrey D Smith Roger Harrison Robert Eisenthal |
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| Institution: | (1) Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Hills Road, CB2 2QR Cambridge, UK;(2) Biochemistry Department, University of Bath, Claverton Down, BA2 7AY Bath, Avon, UK |
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| Abstract: | Substituted enzyme (or ping-pong) mechanisms usually involve enzymes that exist in two forms that alternate during the catalytic
reaction. A method is described here for determining the position of the equilibrium of a half reaction in a ping-pong enzyme
mechanism that is based on the kinetics of the burst reaction which occurs upon addition of reactants that recycle the enzyme
from one form to another. The theoretical basis for the analysis is developed, and the method is applied to the half reaction
of the aldimine form of aspartate transaminase with difluoro-oxaloacetate.
Special issue dedicated to Herman Bachelard |
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| Keywords: | Equilibrium ping-pong mechanism burst kinetics aspartate transaminase |
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