The Involvement of Upregulation and Translocation of Phospho-Rb in Early Neuronal Apoptosis Following Focal Cerebral Ischemia in Rats |
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Authors: | Ying Yu Xiang Luo Qing-Guo Ren Chen-Ju Yi Zhi-Yuan Yu Xue-Wei Xie Wei Wang |
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Institution: | (1) Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People’s Republic of China;(2) Department of Neurology, Hongqi Hospital, Mudanjiang Medical University, Mudanjiang, 157011, People’s Republic of China |
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Abstract: | The aim of this study was to investigate the temporal and spatial relationship between phospho-Rb (ser 795) and neuronal apoptotic
death in rats subjected to transient focal cerebral ischemia. We found increased phosphorylation of Rb and translocation from
neuronal nucleus to cytoplasm in the penumbra zone at 12 h, 1 day, 3 days and 7 days after middle cerebral artery occlusion
(MCAO)/reperfusion, compared with sham-operated controls. At 12 h and 1 day, phospho-Rb appeared to be colocalizated with
TUNEL staining in neurons, but staining was not colocalizated at 3 days and 7 days. These results demonstrated that cytoplasmic
translocation of phospho-Rb from nucleus of neurons occurs in potential apoptotic neurons in the early stages of ischemia/reperfusion,
suggesting that the Rb pathway may only be involved in early neuronal apoptosis and may be not an apoptotic signal in the
late stages of transient cerebral ischemia.
Ying Yu and Xiang Luo contributed equally to this work. |
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Keywords: | MCAO/reperfusion Neuron Early apoptosis Phospho-Rb |
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