Administration of Histidine to Female Rats Induces Changes in Oxidative Status in Cortex and Hippocampus of the Offspring |
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Authors: | Denise Bertin Rojas Tanise Gemelli Rodrigo Binkowski de Andrade Aline Guimarães Campos Carlos Severo Dutra-Filho Clóvis Milton Duval Wannmacher |
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Institution: | 1.Programa de Pós-Gradua??o em Ciências Biológicas: Bioquímica, Departamento de Bioquímica,Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil |
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Abstract: | Histidinemia is an inherited metabolic disorder biochemically characterized by high concentrations of histidine in biological
fluids. Usually affected patients are asymptomatic although some individuals have mental retardation and speech disorders.
Considering the high prevalence of histidinemia and the scarce information on the effects of maternal histidinemia on their
progeny, we investigated various parameters of oxidative stress in brain cortex and hippocampus of the offspring from female
rats that received histidine (0.5 mg/g of body weight) in the course of pregnancy and lactation. At 21 days of age we found
a significant increase of thiobarbituric acid reactive substances (TBARS), 2′,7′-dihydrodichlorofluorescein oxidation, superoxide
dismutase (SOD) activity, catalase (CAT) activity, total sulfhydryls and glutathione (GSH) content in cerebral cortex and
hippocampus. We also verified that at 60 days of age, GSH, SOD and total sulfhydryls returned to normal levels in brain cortex,
while the other parameters decreased in the same structure. In the hippocampus, at 60 days of age GSH returned to normal levels,
CAT persisted elevated and the other parameters decreased. These results indicate that histidine administration to female
rats can induce oxidative stress in the brain from the offspring, which partially recovers 40 days after breastfeeding stopped. |
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