EGb761 Blocks MPP+-Induced Lipid Peroxidation in Mouse Corpus Striatum |
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| Authors: | Rojas Patricia Garduño Belén Rojas Carolina Vigueras Rosa María Rojas-Castañeda Julio Ríos Camilo Serrano-Garcia Norma |
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| Institution: | (1) Laboratory of Neurotoxicology, Instituto Nacional de Neurología y Neurocirugía,  Manuel Velasco Suárez SS, Av. Insurgentes Sur No. 3877, C.P. 14269, México, D.F., México;(2) Instituto de Investigaciones Biomédicas, Department of Physiology, Universidad Nacional Autónoma de México Apartado, Postal 70228 C.P. 04510, México, D.F., México;(3) Laboratory of Histomorphology, Instituto Nacional de Pediatría, Av. Insurgentes Sur No. 3700-C, Col. Insurgentes Cuicuilco, C.P. 04530, México, D.F., México;(4) Department of Neurochemistry, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez SS, Av. Insurgentes Sur No. 3877, C.P. 14269, México, D.F., México |
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| Abstract: | EGb761 has been suggested to be an antioxidant and free radical scavenger. Excess generation of free radicals, leading to lipid peroxidation (LP), has been proposed to play a role in the damage to striatal neurons induced by 1-methyl-4-phenylpyridinium (MPP+). We investigated the effects of EGb761 pretreatment on MPP+ neurotoxicity. C-57 black mice were pretreated with EGb761 for 17 days at different doses (0.63, 1.25, 2.5, 5 or 10 mg/kg) followed by administration of MPP+, (0.18, 0.36 or 0.72 mg/kg). LP was analyzed in corpus striatum at 30 min, 1 h, 2 h and 24 h after MPP+ administration. Striatal dopamine content was analyzed by HPLC at the highest EGb761 dose at 2 h and 24 h after MPP+ administration. MPP+-induced LP was blocked (100%) by EGb761 (10 mg/kg). Pretreatment with EGb761 partially prevented (32%) the dopamine-depleting effect of MPP+ at 24 h. These results suggest that supplements of EGb761 may be effective at preventing MPP+-induced oxidative stress. |
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| Keywords: | MPP+ lipid peroxidation neuroprotection parkinson's disease EGb761 |
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