Metal cations as synaptosomal calcium blockers in studies with Fura-2 |
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Authors: | Ya-Ding Sun Christina G Benishin |
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Institution: | (1) Present address: Dept. of Physiology, University of Alberta Faculty of Medicine, T6G 2H7 Edmonton, Alberta |
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Abstract: | Metal ions are often used to block calcium channels in various tissues, including synaptosomes. In the present study, Fura-2 was used to determine the effectiveness of various metal ions as calcium channel blockers in rat brain synaptosomes in vitro. In buffer solutions, La3+ and Cd2+ increased the Fura-2 fluorescence in a manner similar to Ca2+. Ni2+ and Mn2+ appeared to be fluorescence quenching cations, and Sr2+ and Co2+ had little effect on the fluorescence of Fura-2. In suspensions of synaptosomes under resting conditions, Cd2+, Ni2+ and Mn2+ were found to be not suitable for use in synaptosome studies. On the other hand, La3+ and Co2+ had little effect on the Fura-2 fluorescence of resting synaptosomes, and under depolarizing conditions, La3+ and Co2+ decreased the Fura-2 fluorescence. These resuls, therefore, suggest that La3+ and Co2+ may be suitable as calcium channel blockers in synaptosome studies. |
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Keywords: | Synaptosomes calcium Fura-2 lanthanum cobalt |
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