Quantification of Superoxide Radical in the Brain of Rats with Experimentally Induced Obstructive Jaundice |
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Authors: | Dimitris Konstantinou Adamantios Mavrakis Konstantinos Grintzalis Ioannis Papapostolou Stelios F Assimakopoulos Elisabeth Chroni Christos Georgiou |
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Institution: | (1) Department of Neurosurgery, School of Medicine, University of Patras, Rion, Greece;(2) Department of Biology, Division of Genetics, Cell and Developmental Biology, School of Medicine, University of Patras, Rion, Greece;(3) Department of Internal Medicine, School of Medicine, University of Patras, Rion, Greece;(4) Department of Neurology, School of Medicine, University of Patras, University Hospital of Patras, Rion, 26504, Greece |
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Abstract: | The study aimed to directly measure in vivo superoxide radical (O2−) a direct indicator of oxidative stress, in the brain of rats with experimentally induced obstructive jaundice by employing
a new quantitative ultrasensitive fluorescent assay requiring minimum sample. O2− anion is specific for dihydroethidine (DHE) and upon reaction gives a characteristic product, namely 2-OH-ethidium. Ten male
rats underwent laparotomy and were divided into two groups: I, sham operated and II bile duct ligation. Ten days later, following
injection with DHE (a O2− trap), all animals were killed and samples from cerebral cortex, midbrain and cerebellum were removed for analysis. It was
shown that compared to group I, in group II the O2− was increased by 67% in the cerebral cortex and by 37% in the midbrain as a consequence of experimental obstructive jaundice,
while its levels were unaffected in the cerebellum. The data in this experimental obstructive jaundice model imply a region-specific
increase of O2− formation rate, being higher in cerebral cortex, less so in the midbrain and not at all in cerebellum. |
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Keywords: | Brain oxidative stress Obstructive jaundice Experimental hepatic encephalopathy Superoxide radical Dihydroethidine Reactive oxygen species Free radicals |
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