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Tat-DJ-1 Protects Neurons from Ischemic Damage in the Ventral Horn of Rabbit Spinal Cord Via Increasing Antioxidant Levels |
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| Authors: | Woosuk Kim Dae Won Kim Hoon Jae Jeong Dae Young Yoo Hyo Young Jung Sung Min Nam Jong Hwi Kim Jung Hoon Choi Moo-Ho Won Yeo Sung Yoon Seung Myung Moon Soo Young Choi In Koo Hwang |
| Institution: | 1. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea 6. Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangnung-Wonju National University, Gangneung, 210-702, South Korea 2. Department of Biomedical Science, Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, 200-702, South Korea 3. Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, 200-701, South Korea 4. Department of Neurobiology, School of Medicine, Kangwon, National University, Chuncheon, 200-701, South Korea 5. Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong, 445-170, South Korea |
| Abstract: | The DJ-1 gene is highly conserved in diverse species and DJ-1 is known as an anti-oxidative stress factor. In this study, we investigated the neuroprotective effects of DJ-1 against ischemic damage in the rabbit spinal cord. Tat-DJ-1 fusion proteins were constructed to facilitate the penetration of DJ-1 protein into the neurons. Tat-1-DJ-1 fusion protein was administered to the rabbit 30 min after ischemia/reperfusion, and transient spinal cord ischemia was induced by occlusion of the aorta at the subrenal region for 15 min. The administration of Tat-DJ-1 significantly improved the Tarlov score compared to that in the Tat (vehicle)-treated group at 24, 48 and 72 h after ischemia/reperfusion. At 72 h after ischemia/reperfusion, the number of cresyl violet-positive neurons was significantly increased in the Tat-DJ-1-treated group compared to that in the vehicle-treated group. Lipid peroxidation as judged from the malondialdehyde levels was significantly decreased in the Tat-DJ-1-treated group compared to that in the vehicle-treated group. In contrast, superoxide dismutase and catalase levels were significantly increased in the Tat-DJ-1-treated group compared to that in the vehicle-treated group. This result suggests that DJ-1 protects neurons from ischemic damage in the ventral horn of the spinal cord via its antioxidant effects. |
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