Identification of the coding region for the putidaredoxin reductase gene from the plasmid of Pseudomonas putida |
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Authors: | Charles Romeo Naoko Moriwaki Kerry T Yasunobu Irwin C Gunsalus Hideo Koga |
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Institution: | 1. Department of Biochemistry-Biophysics, John A. Burns School of Medicine, Honolulu, Hawaii, USA 2. Department of Biochemistry, School of Chemical Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA 3. Faculty of Pharmaceutical Science, Kyushu University, Fukuraka, Japan
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Abstract: | The first 12 NH2-terminal amino acids of the Pseudomonas putida putidaredoxin reductase were shown to be Met-Asn-Ala-Asn-Asp-Asn-Val-Val-Ile-Val-Gly-Thr. Comparison of these data with the DNA sequence of the BamHI-HindIII 197-base fragment derived from the PstI 2.2-kb fragment obtained from the P. putida plasmid showed that the putidaredoxin reductase gene was downstream from the cytochrome P-450 gene and the intergenic region had the 24-nucleotide sequence TAAACACATGGGAGTGCGTGCTAA. The Shine-Dalgarno sequence GGAG was detected in this region. The initiating triplet for the reductase gene was GTG, which normally codes for valine, but in the initiating codon position codes for methionine. From the amino acid sequence and X-ray data comparisons with other flavoproteins, what appears to be the AMP binding region of the FAD can be recognized in the NH2-terminal portion of the reductase involving residues 5–35. |
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