| Genetic Mapping of Laminaria japonica and L. Iongissima Using Amplified Fragment Length Polymorphism Markers in a ＂Two-Way Pseudo- Testcross＂ Strategy |
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| 作者姓名： | Yuhui Li Yingxia Yang Jidong Liu Xiuliang Wang Tianxiang Gao Delin Duan |
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| 作者单位： | [1]Ocean University of China, Qingdao 266003, China [2]Institute of Oceanology, the Chinese Academy of Sciences, Qingdao 266071, China [3]Ludong University, Yantai 264025, China |
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| 基金项目： | Supported by the NSFC projects （40376049, 30500383）, the Knowledge Innovation Program of the Chinese Academy of Sciences （D. L. Duan） and the Shandong Agricultural Seedstocks Project.Acknowledgements We are grateful to Dr. Yehao Liu （Nanjing University） for help and suggestions in this study. |
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| 摘 要： | With a ＂two-way pseudo-testcross＂ mapping strategy, we applied the amplified fragment length polymorphism （AFLP） markers to construct two moderate density genetic linkage maps for Laminaria. The linkage maps were generated from the 60 progenies of the F1 cross family （Laminaria iongissima Aresch. × L. Japonica Miyabe） with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1：1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3：1 Mendelian segregation ratio. Among the loci with 1：1 segregating ratios, 79 loci were ordered in 14 linkage groups （648.6 cM） of the paternal map, and 72 loci were ordered in 14 linkage groups （601.9 cM） of the maternal map. The average density of loci was approximately 1 per 8 cM. To Investigate the homologies between two parental maps, we used 58 loci segregated 3：1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci-controlling commercially important traits for Laminaria.
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| 关 键 词： | 昆布属 长海带 真海带 遗传连锁图 双向假测交 扩增片段长度多态性 |
| 修稿时间： | 2006-03-152006-09-16 |
Genetic Mapping of Laminaria japonica and L. longissima Using Amplified Fragment Length Polymorphism Markers in a "Two-Way Pseudo-Testcross" Strategy |
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| Authors: | Yuhui Li Yingxia Yang Jidong Liu Xiuliang Wang Tianxiang Gao Delin Duan |
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| Institution: | Ocean University of China;, Qingdao 266003, China;Institute of Oceanology, the Chinese Academy of Sciences;, Qingdao 266071, China;Ludong University;, Yantai 264025, China |
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| Abstract: | With a “two‐way pseudo‐testcross” mapping strategy, we applied the amplified fragment length polymorphism (AFLP) markers to construct two moderate density genetic linkage maps for Laminaria. The linkage maps were generated from the 60 progenies of the F1 cross family (Laminaria longissima Aresch. ×L. japonica Miyabe) with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1:1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3:1 Mendelian segregation ratio. Among the loci with 1:1 segregating ratios, 79 loci were ordered in 14 linkage groups (648.6 cM) of the paternal map, and 72 loci were ordered in 14 linkage groups (601.9 cM) of the maternal map. The average density of loci was approximately 1 per 8 cM. To investigate the homologies between two parental maps, we used 58 loci segregated 3:1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci‐controlling commercially important traits for Laminaria. |
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| Keywords: | amplified fragment length polymorphism makers genetic linkage map Laminaria japonica Laminaria longissima segregation ratio two-way pseudo-testcross strategy |
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