Phototropins and Their LOV Domains:Versatile Plant Blue-Light Receptors |
| |
作者姓名: | Winslow R. Briggs Tong-Seung Tseng Hae-Young Cho Trevor E. Swartz Stuart Sullivan Roberto A. Bogomolni Eirini Kaiserli John M. Christie |
| |
作者单位: | [1]Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, USA [2]Plant Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12, 8QQ UK [3]Department of Chemistry, University of California, Santa Cruz, CA 95064, USA |
| |
基金项目: | Supported by the National Science Foundation, USA, grants MCB 0091384 and 0444504 to WRB, MCB0444390 to RAB, and by the UK Biotechnology and Biological Sciences Research Council, grant BB/C000366/1 to JMC. Publication of this paper is supported by the National Natural Science Foundation of China (30624808) and Science Publication Foundation of the Chinese Academy of Sciences. |
| |
摘 要: | The phototropins phot1 and phot2 are plant blue-light receptors that mediate phototropism, chloroplast movements, stomatal opening, leaf expansion, the rapid Inhibition of hypocotyl growth in etiolated seedlings, and possibly solar tracking by leaves in those species in which It occurs. The phototroplns are plasma membrane-associated hydrophilic proteins with two chromophore domains (designated LOV1 and LOV2 for their resemblance to domains In other signaling proteins that detect light, oxygen, or voltage) in their Nterminal half and a classic serine/threonlne kinase domain in their C-terminal half. Both chromophore domains bind flavin mononucleotide (FMN) and both undergo light-activated formation of a covalent bond between a nearby cystelne and the C(4a) carbon of the FMN to form the signaling state. LOV2-cystelnyl adduct formation leads to the release downstream of a tightly bound amphlpathlc α-helix, a step required for activation of the klnase function. This cysteinyl adduct then slowly decays over a matter of seconds or minutes to return the photoreceptor chromophore modules to their ground state. Functional LOV2 is required for light-activated phosphorylation and for various blue-light responses mediated by the phototroplns. The function of LOV1 is still unknown, although It may serve to modulate the signal generated by LOV2. The LOV domain Is an ancient chromophore module found In a wide range of otherwise unrelated proteins In fungi and prokaryotes, the latter Including cyanobacterla, eubacterla, and archaea. Further general reviews on the phototropins are those by Celaya and Liscum (2005) and Christie and Briggs (2005).
|
关 键 词: | 植物 蓝光受体 LOV域 趋光性 向光素 |
修稿时间: | 2006-08-142006-10-19 |
Phototropins and Their LOV Domains: Versatile Plant Blue-Light Receptors |
| |
Authors: | Winslow R Briggs Tong-Seung Tseng Hae-Young Cho Trevor E Swartz Stuart Sullivan Roberto A Bogomolni Eirini Kaiserli John M Christie |
| |
Institution: | Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, USA;Plant Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12, 8QQ UK;Department of Chemistry, University of California, Santa Cruz, CA 95064, USA |
| |
Abstract: | The phototropins phot1 and phot2 are plant blue‐light receptors that mediate phototropism, chloroplast movements, stomatal opening, leaf expansion, the rapid inhibition of hypocotyl growth in etiolated seedlings, and possibly solar tracking by leaves in those species in which it occurs. The phototropins are plasma membrane‐associated hydrophilic proteins with two chromophore domains (designated LOV1 and LOV2 for their resemblance to domains in other signaling proteins that detect light, oxygen, or voltage) in their N‐terminal half and a classic serine/threonine kinase domain in their C‐terminal half. Both chromophore domains bind flavin mononucleotide (FMN) and both undergo light‐activated formation of a covalent bond between a nearby cysteine and the C(4a) carbon of the FMN to form the signaling state. LOV2‐cysteinyl adduct formation leads to the release downstream of a tightly bound amphipathic α‐helix, a step required for activation of the kinase function. This cysteinyl adduct then slowly decays over a matter of seconds or minutes to return the photoreceptor chromophore modules to their ground state. Functional LOV2 is required for light‐activated phosphorylation and for various blue‐light responses mediated by the phototropins. The function of LOV1 is still unknown, although it may serve to modulate the signal generated by LOV2. The LOV domain is an ancient chromophore module found in a wide range of otherwise unrelated proteins in fungi and prokaryotes, the latter including cyanobacteria, eubacteria, and archaea. Further general reviews on the phototropins are those by Celaya and Liscum (2005) and Christie and Briggs (2005). |
| |
Keywords: | blue-light receptor flavin-cysteinyl adduct LOV domain phototropin phototropism |
本文献已被 维普 等数据库收录! |
|