Theoretical Proof and Empirical Confirmation of a Continuous Labeling Method Using Naturally ^13C-Depleted Carbon Dioxide |
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作者姓名: | Weixin Cheng Feike A. Dijkstra |
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作者单位: | Environmental Studies, University of California, Santa Cruz, CA 95064, USA |
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基金项目: | 国家自然科学基金;中国科学院资助项目 |
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摘 要: |
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关 键 词: | 二氧化碳浓度 同位素连续标记 同位素示踪 自然损耗 根际 碳通量 |
修稿时间: | 2006-09-022006-09-12 |
Theoretical Proof and Empirical Confirmation of a Continuous Labeling Method Using Naturally 13C-Depleted Carbon Dioxide |
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Authors: | Weixin Cheng Feike A Dijkstra |
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Institution: | Environmental Studies, University of California;, Santa Cruz, CA 95064, USA |
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Abstract: | Continuous Isotope labeling and tracing is often needed to study the transformation, movement, and allocation of carbon in plant-soil systems. However, existing labeling methods have numerous limitations. The present study Introduces a new continuous labeling method using naturally ^13C-depleted CO2. We theoretically proved that a stable level of ^13C-CO2 abundance in a labeling chamber can be maintained by controlling the rate of CO2-free air Injection and the rate of ambient airflow with coupling of automatic control of CO2 concentration using a CO2 analyzer. The theoretical results were tested and confirmed in a 54 day experiment in a plant growth chamber. This new continuous labeling method avoids the use of radioactive ^14C or expensive ^13C-enrlched CO2 required by existing methods and therefore eliminates issues of radiation safety or unaffordable isotope cost, as well as creating new opportunities for short- or long-term labeling experiments under a controlled environment. |
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Keywords: | carbon flux CO2 isotope rhizosphere tracers |
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