普通小麦Qz180中一个抗条锈病基因的分子作图

普通小麦(Triticum aestivum L.)材料Qz180具有良好的抗条锈病特性,经基因推导发现其含有一个优良的抗条锈病的基因,暂定名为YrQz.用Qz180与感病材料铭贤169和WL1分别杂交构建了两个F2群体,用条中30号条锈菌小种对这两个群体进行的抗性测验表明,YrQz为显性单基因遗传.通过SSR和AFLP结合BSA的方法对这个基因进行了分子作图,结果鉴定出与YrQz连锁的2个SSR标记和2个AFLP标记.根据SSR标记的染色体位置,该基因被定位在2B染色体的长臂上,位于两个SSR位点Xgwm388和Xgwm526之间;两个AFLP标记P35M48(452)和P36M61(163)分别位于该基因的两侧,遗传距离分别为3.4 cM和4.1cM.

Identification and Molecular Mapping of a Stripe Rust Resistance Gene from a Common Wheat Line Qz180

YrQz, a stripe rust disease resistance gene was identified in a common wheat (Triticum aestivum L.) line Qz180. This resistance was controlled by a single dominant gene, which was confirmed by genetic analysis of two F₂ populations derived from the crosses using Qz180 and two susceptible parents (Mingxian 169 and WL1). Bulked segregant analysis using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers was conducted in order to map the chromosomal location of YrQz. The results indicated that YrQz was located on the long arm of wheat chromosome 2B and resided in a region flanked by two SSR loci Xgwm388 and Xgwm526. Two AFLP markers P35M48(452) and P36M61 (163) were closely linked to YrQz with the genetic distance of 3.4 cM and 4.1 cM, respectively. To our knowledge, this is the first molecularly mapped stripe rust resistance gene on wheat chromosome 2B.