金丝桃属和三腺金丝桃属植物叶的红外光谱分析及其与分类群鉴定的相关性

利用傅里叶红外光谱仪和OMINI采样器直接迅速准确地测定金丝桃属(Hypericum L.)9组43种1亚种1变种和三腺金丝桃属(Triadenum Raf)2种植物的红外光谱,结果表明:各分类群(种)的红外光谱具有高度特异性和重现性,这两属及其金丝桃属组间的红外光谱图存在较大的差异,而组内种间红外光谱图的差异较小,同种不同分布区和不同发育时期的叶的红外光谱几无差别;其红外光谱图的变化可以作为这两属植物的分类依据之.这也暗示利用已知的标准红外光谱图库,可以区分和鉴定出这两属或其他属植物的种类.

FTIR Spectrum of Hypericum and Triadenum with Reference to Their Identification

The infrared spectroscopic study of leaves of nine sections, 43 species, one subspecies and one variety in Hypericum L. and two species in Triadenum Raf. were conducted directly and rapidly with FTIR and OMNI-sampler accessory. The results showed that the infrared spectra of Hypericum and Triadenum were fingerprint-like patterns which were highly typical for different taxa. Significant differences in the infrared spectra were found between these two genera and among the nine sections of Hypericum. And certain differences exist in the spectra among species in the same section of Hypericum or in the Triadenum. Furthermore, no significant difference was found in the infrared spectral patterns in the leaves at various developmental stages and in leaves of the same species collected from different geographic regions, although occasionally geographic difference did exist in the same species. The results indicated that the infrared spectra of leaves are of taxonomic value at the level of species and sections in these two genera, and this technique can be widely used for identification and classification of other taxa when standard spectra are available.