甜菜碱醛脱氢酶(BADH)基因转化小麦及其表达 |
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引用本文: | 郭北海,张艳敏,李洪杰,杜立群,李银心,张劲松,陈受宜,朱至清.甜菜碱醛脱氢酶(BADH)基因转化小麦及其表达[J].Journal of Integrative Plant Biology,2000,42(3):279-283. |
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作者姓名: | 郭北海 张艳敏 李洪杰 杜立群 李银心 张劲松 陈受宜 朱至清 |
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Transformation of Wheat with a Gene Encoding for the Betaine Aldehyde Dehydrogenase(BADH) |
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Authors: | GUO Bei-Hai ZHANG Yan-Min LI Hong-Jie DU Li-Qun LI Yin-Xin ZHANG Jin-Song CHEN Shou-Yi ZHU Zhi-Qing |
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Abstract: | Betaine aldehyde dehydrogenase (BADH) cDNA cloned from Atriplex hortensis L. in the plasmid pABH9 containing maize ubiquitin promoter and bar gene was transferred into wheat (Triticum aestivum L.) by microprojectile bombardment with 4.1% of average frequency of transformation. From 300 young embryo calli bombarded with the plasmid, 24 transgenic plants were obtained showing BADH gene integration by both PCR and Southern blotting analysis. Among the 24 transgenic plants, 13 exhibited higher BADH activity than the control. Some transgenic plants grew normally with healthy roots on the medium containing 0.7% NaCl while the control plants had very poor roots and finally died. |
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Keywords: | BADH gene transformation microprojectile bombardment wheat |
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