| Construction and Characterization of a cDNA Library from the Pulp of Cara Cara Navel Orange （Citrus sinensis Osbeck） |
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| 作者姓名： | Neng-Guo Tao Juan Xu Yun-Jiang Cheng Xiu-Xin Deng |
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| 作者单位： | State Key Laboratory of Crop Genetic Improvement, National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China （30123001, 30300241, and 30471201）, the High-Tech Research and Development （863） Program of China, and Hubei Provincial Natural Science Foundation of China （2003ABA107）. |
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| 摘 要： | A cDNA library was constructed end characterized from the pulp of Cera Care navel orange （Citrus sinensis Osbeck） at different stages of ripening. Tittering results revealed that approximately 5.086×10^5 independent clones were included in this library. Electrophoresls gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified end the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBenk, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type Ⅲ metallothlonein-llke （MT） gene was observed to occur 13 tlmes, Indlcatlng that the protein may play an important role In frult development and rlpenlng.
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| 关 键 词： | cDNA库 脐橙 柑橘 栽种技术 |
| 收稿时间： | 2005-01-24 |
| 修稿时间： | 2005-01-242005-05-12 |
Construction and Characterization of a cDNA Library from the Pulp of Cara Cara Navel Orange (Citrus sinensis Osbeck) |
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| Authors: | Neng-Guo Tao Juan Xu Yun-Jiang Cheng Xiu-Xin Deng |
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| Institution: | State Key Laboratory of Crop Genetic Improvement, National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | A cDNA library was constructed and characterized from the pulp of Cara Cara navel orange (Citrus sinensis Osbeck) at different stages of ripening. Tittering results revealed that approximately 5.086 × 105 independent clones were included in this library. Electrophoresis gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified and the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBank, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type III metallothionein‐like (MT) gene was observed to occur 13 times, indicating that the protein may play an important role in fruit development and ripening. (Managing editor: Li‐Hui Zhao) |
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| Keywords: | Cara Cara navel orange cDNA library Citrus pulp |
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