| A Putative Calcium-Permeable Cyclic Nucleotide-Gated Channel, CNGC18, Regulates Polarized Pollen Tube Growth |
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| 作者姓名： | Fang Chang An Yan Li-Na Zhao Wei-Hua Wu Zhenbiao Yang |
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| 作者单位： | [1]National Key Laboratory of Plant Physiology and Biochemistry [2]CAU-UCR Joint Center for Biological Sciences, China Agricultural University, Beijing 100094, China [3]Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA |
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| 基金项目： | Supported by a Natural Science Foundation of China grant （0utstanding 0verseas Young Scientist Award # 30428020 to ZY and WW） and a US National Science Foundation grant to ZY. |
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| 摘 要： | A tip-focused Ca^2＋ gradient is tightly coupled to polarized pollen tube growth, and tip-localized influxes of extracellular Ca^2＋ are required for this process. However the molecular identity and regulation of the potential Ca^2＋ channels remains elusive. The present study has implicated CNGC18 （cyclic nucleotide-gated channel 18） in polarized pollen tube growth, because its overexpression induced wider and shorter pollen tubes. Moreover, CNGC18 overexpression induced depolarization of pollen tube growth was suppressed by lower extracellular calcium （Ca^2＋]ex）. CNGC18-yellow fluorescence protein （YFP） was preferentially localized to the apparent post-Golgi vesicles and the plasma membrane （PM） in the apex of pollen tubes. The PM localization was affected by tip-localized ROP1 signaling. Expression of wild type ROP1 or an active form of ROP1 enhanced CNGC18-YFP localization to the apical region of the PM, whereas expression of RopGAP1 （a ROP1 deactivator） blocked the PM localization. These results support a role for PM-Iocalized CNGC18 in the regulation of polarized pollen tube growth through its potential function in the modulation of calcium influxes.
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| 关 键 词： | 尖端生长 钙离子通道 花粉 植物 |
| 修稿时间： | 2007-05-042007-05-10 |
A Putative Calcium-Permeable Cyclic Nucleotide-Gated Channel, CNGC18, Regulates Polarized Pollen Tube Growth |
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| Authors: | Fang Chang An Yan Li-Na Zhao Wei-Hua Wu Zhenbiao Yang |
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| Institution: | ( National Key Laboratory of Plant Physiology and Biochemistry;;CAU-UCR Joint Center for Biological Sciences, China Agricultural University, Beijing 100094, China;;Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA) |
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| Abstract: | A tip-focused Ca2+ gradient is tightly coupled to polarized pollen tube growth, and tip-localized influxes of extracellular Ca2+ are required for this process. However the molecular identity and regulation of the potential Ca2+ channels remains elusive.The present study has implicated CNGC18 (cyclic nucleotide-gated channel 18) in polarized pollen tube growth, because its overexpression induced wider and shorter pollen tubes. Moreover, CNGC18 overexpression induced depolarization of pollen tube growth was suppressed by lower extracellular calcium (Ca2+]ex). CNGC18-yellow fluorescence protein (YFP)was preferentially localized to the apparent post-Golgi vesicles and the plasma membrane (PM) in the apex of pollen tubes.The PM localization was affected by tip-localized ROP1 signaling. Expression of wild type ROP1 or an active form of ROP1enhanced CNGC18-YFP localization to the apical region of the PM, whereas expression of RopGAP1 (a ROP1 deactivator)blocked the PM localization. These results support a role for PM-localized CNGC18 in the regulation of polarized pollen tube growth through Its potential function in the modulation of calcium influxes. |
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| Keywords: | tip growth ROP GTPase R1C3 R1C4 calcium channel |
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