| Use of Random Amplified Polymorphic DNA Analysis for Economically Important Food Crops |
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| 作者姓名： | Halima Hassan Salem Bahy Ahmed Ali Tian-Hua Huang Da-Nian Qin Xiao-Mei Wang Qing-Dong Xie |
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| 作者单位： | [1]Cell Biology and Medical Genetics Department, Medical College, Shantou University, Shantou 515031 [2]Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute,Mubarak City for Scientific Research ＆ Technology Applications, New Borg El-Arab City 21934, Alexandria, Egypt [3]Physiology Department, Medical College, Shantou University, Shantou 515031, China [4]Department of Biochemistry and Molecular Biology, Life Science School, Shenzhen University, Shenzhen 518060, China |
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| 摘 要： | The objective of this review is to summarize numerous studies on the use of the random amplified polymorphic DNA （RAPD） technique on rice, corn, wheat, sorghum, barley, rye, and oats to examine its feasibility and validity for assessment of genetic variation, population genetics, mapping, linkage and marker assisted selection, phylogenetic analysis, and the detection of somaclonal variation. Also we discuss the advantages and limitations of RAPD. Molecular markers have entered the scene of genetic improvement in different fields of agricultural research. The simplicity of the RAPD technique made it ideal for genetic mapping, plant and animal breeding programs, and DNA fingerprinting, with particular utility in the field of population genetics.
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| 关 键 词： | 多形态性 DNA 食用作物 禾本科 |
| 收稿时间： | 2006-09-23 |
| 修稿时间： | 2006-10-19 |
Use of Random Amplified Polymorphic DNA Analysis for Economically Important Food Crops |
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| Authors: | Halima Hassan Salem Bahy Ahmed Ali Tian-Hua Huang Da-Nian Qin Xiao-Mei Wang Qing-Dong Xie |
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| Institution: | (Cell Biology and Medical Genetics Department, Medical College, Shantou University;, Shantou 515031, China;Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research &Technology Applications;, New Borg El-Arab City 21934, Alexandria, Egypt;Physiology Department, Medical College, Shantou University;, Shantou 515031, China;Department of Biochemistry and Molecular Biology, Life Science School, Shenzhen University;, Shenzhen 518060, China) |
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| Abstract: | The objective of this review is to summarize numerous studies on the use of the random amplified polymorphic DNA (RAPD) technique on rice, corn, wheat, sorghum, barley, rye, and oats to examine its feasibility and validity for assessment of genetic variation, population genetics, mapping, linkage and marker assisted selection, phylogenetic analysis, and the detection of somaclonal variation.Also we discuss the advantages and limitations of RAPD.Molecular markers have entered the scene of genetic improvement in different fields of agricultural research.The simplicity of the RAPD technique made it ideal for genetic mapping, plant and animal breeding programs, and DNA fingerprinting, with particular utility in the field of population genetics. |
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| Keywords: | random amplified polymorphic DNA food crops poaceae |
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