Characterization of the Hemolytic Properties of an Extract from Phaeocystis globosa Scherffel |
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作者姓名: | Xi-ChunPENG Wei-DongYANG Jie-ShengLIU Zhi-YingPENG Song-HuiLUE Wen-ZhengDING |
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作者单位: | [1]CollegeofFoodandBiologyEngineering,SouthChinaUniversityofTechnology,Guangzhou510640,China [2]DepartmentofBiotechnology,JinanUniversity,Guangzhou510632,China |
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摘 要: | Phaeocystis globosa Scherffel, an organism that causes harmful algal blooms, is a genus of the family Prymnesiophyta (or Haptophyta) with eurythermal and euryhaline characteristics. P. globosa has been confirmed to produce hemolytic substances, which are a mixture of liposaccharides. In the present study, the hemolytic properties of extract of P. globosa are analyzed further. The effects of temperature, pH,different divalent cations, and membrane lipids on extract-induced hemolysis are discussed, as is the possible hemolytic mechanism. The results of the present study showed that the hemolytic activity of the extract was approximately 127.1 hemolytic units (HU)/L. The hemolytic reaction became fastest and a 50% decrease in absorbance was induced at 30 min at 37℃, and at pH 7.0; Hg^2 was the strongest inhibitor of the hemolysis compared with the other divalent cations and many membrane lipids, except for phosphatidic acid, inhibited the hemolytic activity to different degrees. These results suggest that the toxin may make pores in the surface of red blood cells and that Hg^2 either combines with the hemolysin or closes the pores,hence inhibiting its further hemolytic reaction. The toxin probably has no specific membrane receptor in the red blood cell membrane.
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关 键 词: | 溶血特性 毒素 提取 棕囊藻 脂多糖 |
Characterization of the Hemolytic Properties of an Extract from Phaeocystis globosa Scherffel |
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Authors: | Xi-Chun PENG Wei-Dong YANG Jie-Sheng LIU Zhi-Ying PENG Song-Hui LÜ Wen-Zheng DING |
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Institution: | College of Food and Biology Engineering, South China University of Technology;, Guangzhou 510640, China; Department of Biotechnology, Jinan University;, Guangzhou 510632, China |
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Abstract: | Abstract: Phaeocystis globosa Scherffel, an organism that causes harmful algal blooms, is a genus of the family Prymnesiophyta (or Haptophyta) with eurythermal and euryhaline characteristics. P. globosa has been confirmed to produce hemolytic substances, which are a mixture of liposaccharides. In the present study, the hemolytic properties of extract of P. globosa are analyzed further. The effects of temperature, pH, different divalent cations, and membrane lipids on extract-induced hemolysis are discussed, as is the possible hemolytic mechanism. The results of the present study showed that the hemolytic activity of the extract was approximately 127.1 hemolytic units (HU)/L. The hemolytic reaction became fastest and a 50% decrease in absorbance was induced at 30 min at 37 °C, and at pH 7.0; Hg2+ was the strongest inhibitor of the hemolysis compared with the other divalent cations and many membrane lipids, except for phosphatidic acid, inhibited the hemolytic activity to different degrees. These results suggest that the toxin may make pores in the surface of red blood cells and that Hg2+ either combines with the hemolysin or closes the pores, hence inhibiting its further hemolytic reaction. The toxin probably has no specific membrane receptor in the red blood cell membrane. (Managing editor: Ya-QinHAN) |
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Keywords: | hemolytic liposaccharides Phaeocystis globosa Scherffel toxin |
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