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| 毛竹β-1,4-糖苷酶基因cDNA克隆与表达分析 | |
| 引用本文： | 吴林森,金晓春,杨勇春,黄海松,陈世通,姚伟峰,姚丰平.毛竹β-1,4-糖苷酶基因cDNA克隆与表达分析[J].生物技术通报,2010(3). |
| 作者姓名： | 吴林森 金晓春 杨勇春 黄海松 陈世通 姚伟峰 姚丰平 |
| 作者单位： | 1. 丽水职业技术学院,丽水,323000 2. 丽水市林业局,丽水,323000 3. 德清县林业局,德清,313200 4. 庆元县林业局,庆元,323800 |
| 摘 要： | 根据水稻β-1,4-糖苷酶(korrigan)基因的保守区序列设计引物,以毛竹cDNA为模板,采用PCR方法,成功扩增出1个含有完整阅读框架的cDNA序列,长度为2191bp,共编码617个氨基酸,将其命名为PeKOR基因。其氨基酸序列分析的结果表明,PeKOR与其他β-1,4-糖苷酶有较高的同源性,同水稻序列相似性高达91%,且其序列具有典型的Glycosyl hydrolase9super family结构域,推测此PeKOR为毛竹β-1,4-糖苷酶基因。在竹笋中采用半定量方法研究该基因的表达情况,结果表明该基因在高温条件下表达量较低温条件下明显升高。 |
| 关 键 词： | 毛竹 β-1 4-糖苷酶 克隆 表达分析 |
cDNA Cloning and Expression Analysis of Phyllostachys edulis Beta-1,4-Glycosidase Gene |
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| Wu Linsen,Jin Xiaochun,Yang Yongchun,Huang Haisong,Chen Shitong,Yao Weifeng,Yao Fengping.cDNA Cloning and Expression Analysis of Phyllostachys edulis Beta-1,4-Glycosidase Gene[J].Biotechnology Bulletin,2010(3). | |
| Authors: | Wu Linsen Jin Xiaochun Yang Yongchun Huang Haisong Chen Shitong Yao Weifeng Yao Fengping |
| Institution: | Wu Linsen1 Jin Xiaochun1 Yang Yongchun1 Huang Haisong1 Chen Shitong2 Yao Weifeng3 Yao Fengping4(1 Lishui Vocational Technological College,Lishui 323000,2 Forestry Bureau of Lishui City,3 Forestry Bureau of Deqing,Deqing 313200,4 Forestry Bureau of Qingyuan,Qingyuan 323800) |
| Abstract: | A beta-1,4-glycosidase protein gene from phyllostachys edulis,named as PeKOR,was cloned through PCR using primers designed according to the beta-1,4-glycosidase protein genes conserved region of other plant.The coding region of the genomic clone of PeKOR is continuous.The cDNA of PeKORcontains an open reading frame of 2 191 bp and codes for a protein of 617 aa.The database search using the amino acid sequence as query showed high homology to several beta-1,4-glycosidase proteins,especially the PeKOR sequenc... |
| Keywords: | Phyllostachys pubescens Beta-14 glycosidase protein Cloning Expression analysis |
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