| 洋葱细胞质雄性不育系70及其保持系71基因组DNA的RAPD分析 |
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| 引用本文： | 李园园,王忠,梁峰,沈丽珍,严继勇,杨清.洋葱细胞质雄性不育系70及其保持系71基因组DNA的RAPD分析[J].生物技术通报,2006(6):100-102. |
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| 作者姓名： | 李园园 王忠 梁峰 沈丽珍 严继勇 杨清 |
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| 作者单位： | 1. 南京农业大学生命科学学院,南京,210095
2. 江苏省农业科学院蔬菜研究所,南京,210014 |
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| 基金项目： | 科技部作物转基因专项基金 |
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| 摘 要： | 采用RAPD技术,对洋葱细胞质雄性不育系70及其保持系71的基因组DNA的多态性进行分析。结果表明,在被检测的100条随机引物中,46条引物具有扩增产物,5条引物扩增出多态性条带。对这5条引物进行重复筛选和单株验证,只有G02扩增出的多态性片段,且表现稳定。推测该标记与育性有关。
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| 关 键 词： | 洋葱 细胞质雄性不育 基因组DNA RAPD |
| 收稿时间： | 2006-07-07 |
| 修稿时间： | 2006年7月7日 |
RAPD Analysis of the Genomic DNA of CMS Line 70 and Its Maintainer Line 71 in Onion |
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| Li Yuanyuan,Wang Zhong,Liang Feng,Shen Lizhen,Yan Jiyong,Yang Qing.RAPD Analysis of the Genomic DNA of CMS Line 70 and Its Maintainer Line 71 in Onion[J].Biotechnology Bulletin,2006(6):100-102. |
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| Authors: | Li Yuanyuan Wang Zhong Liang Feng Shen Lizhen Yan Jiyong Yang Qing |
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| Institution: | College of Life Sciences ,Nanjing A gricutural University ,Nanjing 210095;2.Institute of Vegetable Crops ,Jiangsu Academy of Agricultural Sciences ,Nanjing 210014 |
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| Abstract: | Polymorphism was analysed by RAPD in cytoplasmic male sterile line 70 and its maintainer 71 in onion.The results showed that in 100 random primers,the 46 gave amplified products,in which 5 primers presented polymorphism.To verify the stability of polymorphism,8 plants chosen random from the male sterile line and the maintainer respectively were examined by using the 5 primers.It was found that only the primer G02 presented polymorphism steadily.This result indicated that marker G02 could be associated with the sterility of onion. |
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| Keywords: | Onion Cytoplasmic male-sterility Genomic DNA RAPD |
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