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| 商业化种植的六种基因改良玉米品系的鉴定检测方法 | |
| 引用本文： | 曹际娟,覃文,朱水芳,徐宝梁,陈明生,曹远银.商业化种植的六种基因改良玉米品系的鉴定检测方法[J].生物技术通报,2003(1):23-29. |
| 作者姓名： | 曹际娟 覃文 朱水芳 徐宝梁 陈明生 曹远银 |
| 作者单位： | 1. 沈阳农业大学,沈阳,110161;辽宁出入境检验检疫局,大连,116001 2. 广州出入境检验检疫局,广州,510623 3. 国家质检总局动植物检疫实验所,北京,100029 4. 中国进出口商品检验研究所,北京,100025 5. 辽宁出入境检验检疫局,大连,116001 6. 沈阳农业大学,沈阳,110161 |
| 摘 要： | 以PCR方法鉴定检测六种商业化种植的基因改良玉米 (geneticallymodifiedmaize,简称GM 玉米 )。针对Mon810 (Monsanto公司 )、Bt11(Novartis公司 )、Event176 (Novartis公司 )、CBH 35 1(AgrEvo公司 )、T14/T2 5Liberty (AgrEvo公司 )及GA2 1(Monsanto公司 )GM 玉米转入的外源基因质粒图谱 ,设计具有品系特异性的引物进行PCR检测 ,建立了GM 玉米品系鉴定检测的方法。 |
| 关 键 词： | PCR 基因改良玉米 品系鉴定检测 |
Method for the Identification of Six lines of Genetically Modified Maize |
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| Cao jijuan , Qin wen Zhu shuifang Xu baoliang Chen Mingsheng Cao Yuanyin.Method for the Identification of Six lines of Genetically Modified Maize[J].Biotechnology Bulletin,2003(1):23-29. | |
| Authors: | Cao jijuan Qin wen Zhu shuifang Xu baoliang Chen Mingsheng Cao Yuanyin |
| Institution: | Cao jijuan 1,2 Qin wen3 Zhu shuifang4 Xu baoliang5 Chen Mingsheng2 Cao Yuanyin1 |
| Abstract: | To detect and identify six lines of genetically modified (GM) maize, polymerase chain reaction (PCR) assays were performed in this study. Primers specific for inserted genes in the Event 176 GM maize (Novartis company), Bt11 (Novartis company), Mon810 (Monsanto company), T14/T25 (AgrEvo company), CBH-351 (AgrEvo company) and GA21 (Monsanto company) were used to conduct the PCR assays. PCR method was established to detect and identify lines of GM maize. |
| Keywords: | PCR GM-maize Identification of lines |
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