CHO工程细胞株低血清培养的初探

以DMEM: F12(1: 1)为基础培养基,对CHO细胞的低血清培养进行了初步探索,降低培养基中血清含量,观察了细胞在10%、5%和1%等不同浓度低血清培养条件下生长状态和外源蛋白表达活性的变化.试验结果表明:在含1%血清的F12: DMEM(1: 1)培养基中,细胞仍能保持良好的生长状态,且培养上清中的目的蛋白活性与常规血清浓度10% DMEM培养条件下的活性接近.从而达到了既可以降低生产成本,又可以降低纯化难度的目的.

Primarily Exploring on Serum-low Culture of Engineering Cells of CHO

This experiment has explored the serum-low culture of engineering cells of CHO, without affecting the protein expressed,we used DMEM: F12 (1:1) to culture the cells which serum concentrations from 10% down to 1% , the growth of cells was well. It proved that according to the features of different serum training, mixed these serum training reasonably, the effectiveness of training cells can be improved in some degree. It not only can low the costs of production, but also can simplify the process of sublimating.