| 一种高质量麦冬总RNA的提取方法 |
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| 引用本文： | 刘君,韩烈保,李雪,王月华.一种高质量麦冬总RNA的提取方法[J].生物技术通报,2007(6):101-104. |
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| 作者姓名： | 刘君 韩烈保 李雪 王月华 |
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| 作者单位： | 北京林业大学草坪研究所,北京,100083 |
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| 基金项目： | 国家转基因植物研究与产业化专项项目(J2002B006)，国家863项目(2001AA244082)，“教育部跨世纪优秀人才支持计划”资助 |
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| 摘 要： | 本方法采用CTAB作为去污剂,分别用氯仿/异戊醇反复抽提、LiCl沉淀,以去除蛋白质、碳水化合物和次生代谢物等杂质,用DNase处理去除DNA污染,最后用无水乙醇沉淀获得总RNA。该方法不仅能获得完整性好、纯度高的总RNA,而且操作简单、成本低廉、RNA产率高,对富含次生物质的中草药材植物组织总RNA的提取具有借鉴意义。
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| 关 键 词： | CTAB 麦冬 总RNA提取 RNA纯度 |
| 收稿时间： | 2007-06-05 |
| 修稿时间： | 2007年6月5日 |
A Method of Isolating High Quality Total RNA from Ophiopogon japonicus |
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| Liu Jun Han Liebao Li Xue Wang Yuehua.A Method of Isolating High Quality Total RNA from Ophiopogon japonicus[J].Biotechnology Bulletin,2007(6):101-104. |
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| Authors: | Liu Jun Han Liebao Li Xue Wang Yuehua |
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| Abstract: | This Research was carried out following this process:Ophiopogon japonicus fresh leaves were ground,CTAB was added,after centrifuged,upper liquid was extracted by chloroform/iso-Amyl alcohol,precipitated by Licl in order to get rid of protein,carbohydrate,subordination-metabolite etc,and then digested by DNase to get ride of DNA contamination.Finally,Total RNA was precipitated by ethanol.This method can not only get good integrality,purity RNA,but also operate simply,cost less,produce high-yield total RNA.It also gives better suggestions to extract total RNA of herbal medicine plants which are rich of subordination-substance. |
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| Keywords: | CTAB Ophiopogon japonicus Total RNA extraction Purity of RNA |
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