| 二元融合子与荧光假单胞菌耐药性遗传标记的筛选 |
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| 引用本文： | 陈光荣,肖克宇.二元融合子与荧光假单胞菌耐药性遗传标记的筛选[J].生物技术通报,2006(3):73-76. |
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| 作者姓名： | 陈光荣 肖克宇 |
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| 作者单位： | 1. 暨南大学水生所,广州,510632;湖南农业大学水产系,长沙,410128
2. 湖南农业大学水产系,长沙,410128 |
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| 摘 要： | 应用定性和定量药物敏感性试验与诱变相结合,筛选出荧光假单胞菌56-12-10菌株的遗传标记为链霉素抗性,二元融合子AM-1菌株的遗传标记为环丙沙星抗性,确定了在这两种细菌原生质体融合试验中,选择培养基中链霉素的应用浓度为300Iu/ml,环丙沙星应用浓度为100Iu/ml。
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| 关 键 词： | 二元融合子 荧光假单胞菌 遗传标记 |
| 收稿时间： | 2005-12-29 |
| 修稿时间： | 2005年12月29 |
Selection of Antimicrobial Tolerance Genetic Marker of Protoplast Fusion Between Binary Strain and Pesudomonas fluorescens |
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| Chen Guangrong,Xiao Keyu.Selection of Antimicrobial Tolerance Genetic Marker of Protoplast Fusion Between Binary Strain and Pesudomonas fluorescens[J].Biotechnology Bulletin,2006(3):73-76. |
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| Authors: | Chen Guangrong Xiao Keyu |
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| Institution: | 1.Institute of hydrobiologyjinan university, Guangzhou 510632; 2.Aquatic department of hunan agriculture university, changsha 410128 |
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| Abstract: | Using the qualitative and quantitative antimicrobial susceptibility experiment to screen the genetic markers of protoplast fusion between 56-12-10 strains and AM-1 strains which are Smr(streptomycin resistance) and Cmr(ciprofloxacin resistance)respectively.it can be assured that the applied chroma in selective culture medium of streptomycin and ciprofloxacin are 300 Iu/ml and 100 Iu/ml in the protoplast fusion experiment between two bacteria. |
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| Keywords: | Binary fusion Pesudomonas fluorescens Genetic marker |
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