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| 变色栓菌漆酶基因的克隆与序列分析 | |
| 引用本文： | 雷威,陈立祥,苏建明,张学文,章怀云.变色栓菌漆酶基因的克隆与序列分析[J].生物技术通报,2006(5):90-93. |
| 作者姓名： | 雷威 陈立祥 苏建明 张学文 章怀云 |
| 作者单位： | 1. 湖南农业大学动物科技学院,长沙,410128 2. 湖南农业大学生物科学与技术学院,长沙,410128 3. 中南林业科技大学生命科学与技术学院,长沙,410081 |
| 摘 要： | 本研究以PCR扩增的方法,从变色栓(菌Trametes versicolor)的基因组DNA中扩增出了一预期大小的DNA片断,并将其克隆到了pUCM-T载体上。经筛选、酶切、PCR鉴定,序列分析,证明该片断为变色栓菌漆酶基因的克隆。该基因的开放阅读框由1560个核苷酸组成,编码一个由519个氨基酸组成的多肽。与GeneBank中发表的Laccase基因(AY081188)序列比较发现,编码的氨基酸序列同源性为96%,而核苷酸序列的同源性为92%。 |
| 关 键 词： | 变色栓菌 漆酶基因 克隆 序列分析 |
| 收稿时间： | 2006-03-29 |
| 修稿时间： | 2006年3月29日 |
Cloning and Sequencing of a Laccase Gene from T.versicolor |
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| Lei Wei,Chen Lixiang,Su Jianming,Zhang Xuewen,Zhang Huaiyun.Cloning and Sequencing of a Laccase Gene from T.versicolor[J].Biotechnology Bulletin,2006(5):90-93. | |
| Authors: | Lei Wei Chen Lixiang Su Jianming Zhang Xuewen Zhang Huaiyun |
| Institution: | 1.College of Animal Science and Technology HA U, Changsha 410128; 2.College of Biology Science and Technology HA U, Changsha 410128; 3.College of Life Science and Technology CSFU, Changsha 410081 |
| Abstract: | The Laccase gene of Trametes versicolor's genomic DNA amplified by PCR using a set of primers that we designed in this study.The amplified fragment was cloned into the vector pUCM-T.Through transferring,screening,en-zymolysis and sequencing,it was demonstrated that this fragment was Laccase gene.It contains one open reading frame(ORF),which encodes for a polypeptide containing 519 amino acids.The Trametes versicolor Laccase gene has a high ho-mology with other Laccase gene(AY 081188). |
| Keywords: | Trametes versicolor Cloning Laccase gene Sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |