| 毕赤酵母外源基因表达系统研究进展 |
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| 引用本文： | 杜中军,朱水芳,黄文胜,翟衡.毕赤酵母外源基因表达系统研究进展[J].生物技术通报,2002(4):7-11. |
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| 作者姓名： | 杜中军 朱水芳 黄文胜 翟衡 |
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| 作者单位： | 1. 山东农业大学园艺学院,泰安,271018
2. 国家动植物检疫实验所,北京,100029 |
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| 摘 要： | 巴斯德毕赤酵母Pichia pastoris外源基因表达系统已经成功表达了很多胞间型和胞内型蛋白质。与酿酒酵母Saccharomyces ceresiviae相比，该系统所具有的很多优势使其应用越来越广泛。有关研究主要涉及以下几个方面：宿主菌株，表达载体，转化方法，外源基因整合，外源蛋白糖基化和高密度发酵培养等。
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| 关 键 词： | 毕赤酵母 外源基因表达系统 研究进展 甲醇营养型酵母 |
Advances in the Pichia Pastoris Hererologous Gene Expression System |
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| Du Zhongjun,Zhu Shuifang,Huang Wensheng,Zhai Heng.Advances in the Pichia Pastoris Hererologous Gene Expression System[J].Biotechnology Bulletin,2002(4):7-11. |
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| Authors: | Du Zhongjun Zhu Shuifang Huang Wensheng Zhai Heng |
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| Institution: | Du Zhongjun 1 Zhu Shuifang 2 Huang Wensheng 2 Zhai Heng 1 |
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| Abstract: | Pichia pastoris hererologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest. It will be employed more widely for its many advantages over Saccharomyces ceresiviae. Its many features are described here. These features include: the host strains, the expression vectors, the transformation methods, and the integration of the interest gene into the genome of P.pastoris, the glycosylation of the heterologous protein and high-density fermentation and others. |
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| Keywords: | Methylotrophic yeasts Pichia pastoris Expression system |
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