一株溶藻细菌NP23的初步分离鉴别及其溶藻作用研究

从水体中分离得到一株具有溶藻能力的细菌,命名为NP23。经形态特征、生理生化鉴定和16S rDNA序列分析表明,该菌株属于肠杆菌属(Enterobacter)。研究了该菌株对湖泊中优势藻的溶藻效果,初步探讨了其溶藻方式及溶藻物质。结果表明,该菌株对小球藻、惠氏微囊藻、栅藻和蛋白核小球藻具有一定的去除效果,叶绿素a的去除率分别为64.1%、53.1%、87.2%和84.4%,而且在10-108CFU/mL菌浓度范围内,藻的去除率与菌液的浓度成正相关;该菌株对小球藻、栅藻和蛋白核小球藻是间接溶藻,对惠氏微囊藻是直接溶藻;该菌株对栅藻的溶藻物质是蛋白类物质,对蛋白核小球藻的溶藻因子是菌体胞外分泌的具有热稳定性的非蛋白类物质。

Isolation, Identification and Function of an Algicidal Bacteria Strain

One strain of algicidal bacteria NP23 was isolated from water.Its morphology characteristic,physiological biochemistry characteristic and the results of 16S rDNA sequence analyze strongly suggested that algicidal bacteria NP23 belongs to Enterobacter.Focusing on its algicidal effect and mechanism against advantage algaes from Lakes.Results showed that the algicidal bacteria NP23 had a good removal effect on Chlorella,Microcystis wesenbergii,Scenedesmus and Chlorella pyrenoidosa.The removal rates to chlorophyll a reached 64.1%,53.1%,87.2% and 84.4%,and in the filtrate concentration ranges of 10-108 CFU/mL,its removal rates and filtrate concentration showed positive correlation.NP23 algicidal mechanism is direct dissolve algae of Chlorella,Scenedesmus and Chlorella pyrenoidosa and indirect dissolve algae of Microcystis wesenbergii.The dissolved algae matter produced by this strain was of protein substances of Scenedesmus and of extracellular thermo-stable non-protein substances of Chlorella pyrenoidosa.