| 苦荞谷胱甘肽转移酶(FtGST)基因的克隆与序列分析 |
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| 引用本文： | 赵海霞,李双江,李成磊,陈惠,吴琦.苦荞谷胱甘肽转移酶(FtGST)基因的克隆与序列分析[J].生物技术通报,2012(7):70-76. |
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| 作者姓名： | 赵海霞 李双江 李成磊 陈惠 吴琦 |
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| 作者单位： | 四川农业大学生命科学与理学院,雅安,625014 |
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| 基金项目： | 四川农业大学引进人才项目 |
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| 摘 要： | 采用RACE技术,从苦荞(Fagopyrum tatarium)中克隆得到一个谷胱甘肽转移酶(Glutathione S-transferase protein,FtGST)基因。序列分析表明,FtGST基因全长DNA序列和cDNA序列编码区分别为746 bp和666 bp,DNA序列含有一个长度为80 bp(342-421 bp)的内含子;开放阅读框(ORF)长666 bp,编码221个氨基酸。生物信息学分析表明,FtGST基因推导的蛋白质含有Tau家族典型的底物结合口袋、谷胱甘肽结合位点(G-site)和疏水性底物结合位点(H-site)氨基酸残基,表明FtGST为Tau家族蛋白。
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| 关 键 词： | 苦荞 谷胱甘肽转移酶 基因克隆 序列分析 |
Cloning and Sequence Analysis of the Glutathione S-transferase (FtGST)Gene from Fagopyrum tatarium |
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| Zhao Haixia
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Li Shuangjiang
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Li Chenglei
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Chen Hui
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Wu Qi.Cloning and Sequence Analysis of the Glutathione S-transferase (FtGST)Gene from Fagopyrum tatarium[J].Biotechnology Bulletin,2012(7):70-76. |
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| Authors: | Zhao Haixia Li Shuangjiang Li Chenglei Chen Hui Wu Qi |
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| Institution: | Zhao Haixia Li Shuangjiang Li Chenglei Chen Hui Wu Qi(College of Life Science,Sichuan Agricultural University,Ya’an 625014) |
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| Abstract: | A Glutathione S-transferase(FtGST)gene was isolated from Fagopyrum tataricum by RACE method.Sequence analysis showed that the full-length DNA and cDNA of FtGST was 746 bp and 666 bp,respectively.The DNA of FtGST contains one intron,length of 80 bp(342-421 bp).The open reading frame(ORF)of FtGST encodes 221 amino acid residues.Bioinformatics analysis showed that FtGST protein contains some typical amino acid residues of Tau class to constitute substrate binding pocket,glutathione-binding site(G-site)and hydrophobic substrate binding site(H-site).All the characterizations prove FtGST belongs to the Tau proteins family. |
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| Keywords: | Fagopyrum tatarium Glutathione S-transferase(GST) Gene cloning Sequence analysis |
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