微重力条件下心肌细胞在聚羟基乙酸纤维支架上的三维固定化培养

以 1d龄Wistar乳鼠的心室肌组织为心肌细胞的来源 ,采用胰蛋白酶消化及细胞差速贴壁分离心肌细胞 ,以未经修饰和经鼠尾胶原溶液浸泡修饰的聚羟基乙酸 (polyglycolicacid ,PGA)纤维支架作为心肌细胞体外三维 (3D)固定化培养的支架 ,比较心肌细胞在静置培养体系及微重力培养体系下的生长、形态和收缩状况。心肌细胞在未经处理的PGA纤维支架 3D固定化培养时 ,心肌细胞在其上的分布不均匀 ,大部分心肌相互连接形成球状聚集体 ;PGA纤维支架经鼠尾胶原溶液浸泡处理后 ,心肌细胞在其上的分布较为均匀 ,细胞形态多呈梭形或不规则状 ,心肌细胞自律性搏动的幅度加大。在模拟微重力培养条件下 ,心肌细胞在经鼠尾胶原溶液浸泡修饰的PGA纤维支架上的分布更为均匀 ,心肌细胞形成具有自律性同步收缩特性、面积约为 15mm2 的类组织样 3D结构

Three-dimensional Cultivation of Cardiac Cells on Fibrous Polyglycolic Acid Scaffolds in Simulated Microgravity

Cardiac cells were isolated from neonatal rat ventricles by enzymatic digestiontrypsin digestion and different paste speed of cells. The growth, morphology and contractile function of cardiac cells cultured both on fibrous polyglycolic acid (PGA) scaffolds and collagen modified fibrous PGA scaffolds in static system and simulated microgravity system were investigated. Inoculation of cardiac cells on fibrous PGA scaffolds resulted in an uneven distribution of cardiac cells and most of the cardiac cells formed spherical aggregates. Three dimensional cultivation of cardiac cells on collagen modified fibrous PGA scaffolds resulted in a more even distribution of cardiac cells with intense rhythmical contractile function. Furthermore, cardiac cells cultured on collagen modified fibrous PGA scaffolds formed extensive three dimensional aggregates that were maintained structural integrity and contained spontaneously and rhythmically macroscopic contractile areas (approximately 15 mm 2) in unison in simulated microgravity.