| 藏红花细胞悬浮培养动力学研究 |
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| 引用本文： | 陈书安,王晓东,袁晓凡,赵兵,王玉春.藏红花细胞悬浮培养动力学研究[J].生物技术通报,2011(4). |
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| 作者姓名： | 陈书安 王晓东 袁晓凡 赵兵 王玉春 |
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| 作者单位： | 1. 中国科学院过程工程研究所生化工程国家重点实验室,北京,100190;中国生物技术发展中心,北京,100036
2. 中国科学院过程工程研究所生化工程国家重点实验室,北京,100190 |
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| 基金项目： | 中国科学院"西部行动高新技术项目" |
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| 摘 要： | 基于已经建立的藏红花细胞悬浮培养体系,研究了其悬浮培养动力学.结果表明,悬浮培养时细胞的生长周期约为20 d,在20 d时生物量达到最大,为12.3 g DW/L,但是藏红花素的合成周期大约为28 d,在28 d时藏红花素的含量和产量达到最大,分别为95.8 mg/g和0.92 g/L.藏红花素的积累与细胞的生长之间的关系为半生长偶联型,为其生物反应器放大培养提供了参数等理论依据.
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| 关 键 词： | 藏红花 悬浮 培养体系 动力学 生物反应器 |
Investigation on the Kinetics of Crocus sativus L.Cell in Suspension Culture System |
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| Chen Shu-an,Wang Xiaodong,Yuan Xiaofan,Zhao Bing,Wang Yuchun.Investigation on the Kinetics of Crocus sativus L.Cell in Suspension Culture System[J].Biotechnology Bulletin,2011(4). |
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| Authors: | Chen Shu-an Wang Xiaodong Yuan Xiaofan Zhao Bing Wang Yuchun |
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| Institution: | Chen Shu-an1,2 Wang Xiaodong1 Yuan Xiaofan1 Zhao Bing1 Wang Yuchun1(1National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,2The China National Center for Biotechnology Development,Beijing 100036) |
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| Abstract: | Based on the established cell suspension culture system of Crocus sativus L.,its kinetics was investigated.The highest biomass reached 12.3 g DW/L on 20th d,and the highest crocin content and production were obtained on 28th d,both of which were 95.8 mg/g and 0.92 g/L,respectively.The process of croin biosynthesis was semi-associated with the cell growth of Crocus sativus L..This research could provide references for the scale-up culture of Crocus sativus L.cell by bioreactor. |
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| Keywords: | Crocus sativus L Suspension Culture system Kinetics Bioreactor |
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