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| microRNA的实验和生物信息学开发方法 | |
| 引用本文： | 王晶,朱荣胜,宋万坤,张闻博,刘春燕,胡国华,陈庆山.microRNA的实验和生物信息学开发方法[J].生物技术通报,2008(Z1). |
| 作者姓名： | 王晶 朱荣胜 宋万坤 张闻博 刘春燕 胡国华 陈庆山 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨,150030 2. 东北农业大学理学院,哈尔滨,150030 3. 东北农业大学农学院,哈尔滨,150030;黑龙江省农垦科研育种中心,哈尔滨,150090 4. 黑龙江省农垦科研育种中心,哈尔滨,150090;国家大豆工程技术研究中心,哈尔滨,150050 |
| 基金项目： | 黑龙江省“十一五”科技攻关项目(GA06B101-2-6);;国家高技术研究发展计划(863计划)项目(2006AA100104-3);;黑龙江省博士后资助项目(LRB06-126) |
| 摘 要： | mircroRNA是一类约22nt的内源小分子RNA,普遍存在于真核生物体内,它通过与靶基因互补结合来调控基因表达,从而对生物体的生长、发育等起到重要的调节作用。随着生物学技术的不断发展,mircroRNA研究的越发深入,越来越多的mircroRNA被开发出来。本文结合了mircroRNA的生理特点,从RNA、DNA和生物信息学预测这3个方面归纳总结有关开发microRNA的方法,以及由此衍生出来的相关的研究方法。通过对这些研究方法的分类总结,不但为mircroRNA以后的研究工作奠定良好的基础,而且有利于新技术的研发。 |
| 关 键 词： | microRNA 作用机制 开发 同源性 靶基因预测 |
The Method of Development MicroRNAs Based on Experiment and Bioinformation |
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| Wang Jing,Zhu Rongsheng,Song Wankun,Zhang Wenbo,Liu Chunyan,Hu Guohua,Chen Qingshan.The Method of Development MicroRNAs Based on Experiment and Bioinformation[J].Biotechnology Bulletin,2008(Z1). | |
| Authors: | Wang Jing Zhu Rongsheng Song Wankun Zhang Wenbo Liu Chunyan Hu Guohua Chen Qingshan |
| Institution: | 1;2;1;2;2;3;1 Agriculture College;Northeast Agricultural University;Harbin 150030;2 The Crop Research and Breeding Center of Land-Reclamation;Harbin 150090;3 The National Research Center of Soybean Engineering and Technology;Harbin 150050;4 College of Science;Harbin 150030 |
| Abstract: | microRNAs were endogenesis small molecular RNA of 22nt in eukaryotes,and they also played an important role in regulation of development through combining with target gene to regulate gene expression.As the development of biotechnology,the research of microRNA became deeply,more and more microRNAs had been developed.This article summrized the method of developping microRNA though 3 part of RNA,DNA,and bioinformatic prediction and other research method which based on the character of physiology.With this met... |
| Keywords: | microRNA Mechanism Development Homology Prediction of target gene |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |