| 实时荧光定量PCR在猪肺炎支原体检测中的应用 |
| |
| 引用本文： | 王建波,任杰,张映.实时荧光定量PCR在猪肺炎支原体检测中的应用[J].生物技术通报,2009(7). |
| |
| 作者姓名： | 王建波 任杰 张映 |
| |
| 作者单位： | 1. 山西农业大学动物科技学院,太谷,030801
2. 宁夏灵武市畜牧局,灵武,750400 |
| |
| 基金项目： | 山西省科技厅攻关项目 |
| |
| 摘 要： | 实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术,具有高度的灵敏性、特异性和精确性.综述了荧光定量PCR技术的基本原理及其在猪肺炎支原体检测中的应用.
|
| 关 键 词： | 实时荧光定量PCR 猪肺炎支原体 定量检测 |
Application of Real-time Fluorescent Quantitative PCR Technology in the Detection of Mycoplasma hyopneumoniae |
| |
| Wang Jianbo,Ren Jie,Zhang Ying.Application of Real-time Fluorescent Quantitative PCR Technology in the Detection of Mycoplasma hyopneumoniae[J].Biotechnology Bulletin,2009(7). |
| |
| Authors: | Wang Jianbo Ren Jie Zhang Ying |
| |
| Abstract: | Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence,as it features high sensitivity,accuracy,and specificity.In this paper,the basic principle of real-time PCR and its application in the detection of Mycoplasma hyopneumoniae were reviewed. |
| |
| Keywords: | Real-time PCR Mycoplasma hyopneumoniae Quantitative detection |
| 本文献已被 万方数据 等数据库收录！ |
|
