毛木耳种质资源的酯酶同工酶分析

采用垂直平板聚丙烯酰胺凝胶电泳技术对毛木耳56个菌株酯酶同工酶的酶谱多样性进行了研究。结果表明,56份材料有一定的遗传变异,共检测到迁移率不同的10条谱带,各菌株分别具有1~6条酶带,共有26种酶谱类型。菌株被分成了8大群:第一群包括黄耳zh等34个菌株;第二群包括白背木耳等8个菌株;第三、第四群分别为黑木耳和915两个单独的菌株;第五群包括99等4个菌株;第六群包括43等4个菌株;第七群包括50385和AP067两个菌株;第八群包括小上3和杂交34两个菌株。酯酶同工酶分析技术是鉴别种及品种以下菌株的有效手段。

Analysis on Esterase Isozyme of Auricularia. polytricha

Esterase isozyme zymogram polymorphisms of 56 strains in A.polytricha were investigated using vertical slab polyacrylamide gel electrophoresis.The results showed that there existed diversity among coarse muer strains.Seventy-six zymogram bands were observed and there were 26 zymogram types in all tested strains.Each strain had 1~6 bands respectively.Differences of strains were expressed in their respective characteristic zymograms in the study.The results of cluster analysis at 67% similarity level showed that all strains tested could be clustered into 8 groups,i.e.the first group including 34 strains of huang-er zh etc.,the second group including 21 strains of white back moer etc.,the third and the forth group only single strain of A.auricla and 915 respectively,the fifth group including 4 strains of 99 etc.,the sixth group including 4 strains of 43 etc.,the seventh group including 2 strains of 50385 and APO67,the eighth group including 2 strains of xiao-shang 3 and hybrid 34.The analytical technique of esterase isozyme was an available method for identifying strains in species and under species.