| 毕赤酵母Mut+和Muts重组子表达植酸酶基因及其表达物的酶学性质比较 |
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| 引用本文： | 李健,彭远义.毕赤酵母Mut+和Muts重组子表达植酸酶基因及其表达物的酶学性质比较[J].生物技术通报,2006(3):58-62. |
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| 作者姓名： | 李健 彭远义 |
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| 作者单位： | 西南大学动物科学技术学院,重庆,400716 |
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| 摘 要： | 将本实验室已成功构建的植酸酶基因表达载体pPIC9K-phyA酶切线性化后,通过原生质体法整合到巴氏毕赤酵母(Pichia pastoris)GS115菌株细胞中,利用测定酶活、观察转化子在MM和MD平板上的生长情况以及PCR菌落鉴定,筛选出表型为甲醇利用缓慢型的重组子(His Muts)。在相同培养条件下比较甲醇利用缓慢型的重组子和Mut 重组子在表达植酸酶方面的异同。实验结果表明,诱导表达48h后,Mut 和MutS重组子表达的产物在SDS-PAGE胶上都出现了清晰的目的带。酶学性质测定表明,酶学性质无显著差异。
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| 关 键 词： | 植酸酶 MutS重组子 Mut~ 重组子 酶学性质 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005年12月19 |
Expression of Phytase Gene in Pichia Pastoris Muts and Mut+ Recombinants and Enzyme Properties of Phytase |
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| Li jian,Peng Yuanyi.Expression of Phytase Gene in Pichia Pastoris Muts and Mut+ Recombinants and Enzyme Properties of Phytase[J].Biotechnology Bulletin,2006(3):58-62. |
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| Authors: | Li jian Peng Yuanyi |
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| Institution: | College of Animal Science and Technology, Southwest University,Chongqing 400716 |
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| Abstract: | The expression vector pPIC9K including phytase gene was linearized and then transformed into Pichia pastoris GS115 by means of producing plasmatosome.The Muts recombinant was screened by phenotype and PCR and the expression of phytase was compared with Mut recombinant in Pichia pastoris under the same condition.SDS-PAGE results indicated that there was a clear target band in Muts and Mut recombinant Culture supernatant after 48 hours culture respectively.Enzyme properties of tests showed that there was no significant difference between them. |
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| Keywords: | Phytase Muts recombinant Mut recombinant Enzyme properties |
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