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甘薯、胡萝卜发根单寄主培养体系繁殖马铃薯腐烂线虫的研究
引用本文:阮龙,姜疆,吴飞,刘晓锋,马代夫,吴跃进,查向东,王钰.甘薯、胡萝卜发根单寄主培养体系繁殖马铃薯腐烂线虫的研究[J].生物技术通报,2006(6):103-107.
作者姓名:阮龙  姜疆  吴飞  刘晓锋  马代夫  吴跃进  查向东  王钰
作者单位:1. 中国科学院离子束生物工程学重点实验室,合肥,230031;安徽农业大学,合肥,230036
2. 中国科学院离子束生物工程学重点实验室,合肥,230031
3. 安徽大学生命科学院,合肥,230039
4. 安徽科技学院生命科学院,凤阳,233100
5. 江苏徐州甘薯研究中心,徐州,221121
基金项目:国家高技术研究发展计划(863计划);安徽省自然科学基金;安徽省农科院院长青年基金
摘    要:利用发根农杆菌A4转化甘薯品种徐薯18和胡萝卜品种天红2号的发根,建立甘薯茎线虫病病原线虫(马铃薯腐烂线虫)的单寄主培养体系。通过该体系对马铃薯腐烂线虫的行为进行观察以及繁殖情况进行调查。结果表明:(1)马铃薯腐烂线虫在甘薯和胡萝卜发根上都能正常发育和繁殖,完成其生活周期;(2)培养4周和8周后,在甘薯发根上线虫繁殖倍数分别为2.6和50.6倍;在胡萝卜发根上线虫繁殖倍数分别为1.7和9.9倍;相同培养时间内,线虫在甘薯发根上的繁殖数极显著高于在胡萝卜发根上的繁殖数。(3)利用发根系统繁殖马铃薯腐烂线虫,便于研究其行为,在显微镜下可以直接观察到线虫在发根上活动情况,这对研究发根和线虫相互关系十分有利。基于上述结果,初步证实构建甘薯发根单寄主培养体系繁殖马铃薯腐烂线虫是可行的,且优于胡萝卜发根繁殖马铃薯腐烂线虫体系。

关 键 词:马铃薯腐烂线虫  甘薯发根  胡萝卜发根  单寄主培养
收稿时间:2006-08-15
修稿时间:2006年8月15日

Study of Reproduction of Ditylenchus Destructor Under Monoxenic Condition of Sweet Potato and Carrot Hairy Roots
Ruan Long,Jiang Jiang,Wu Fei,Liu Xiaofeng,Ma Daifu,Wu Yuejin,Cha Xiangdong,Wang Yu.Study of Reproduction of Ditylenchus Destructor Under Monoxenic Condition of Sweet Potato and Carrot Hairy Roots[J].Biotechnology Bulletin,2006(6):103-107.
Authors:Ruan Long  Jiang Jiang  Wu Fei  Liu Xiaofeng  Ma Daifu  Wu Yuejin  Cha Xiangdong  Wang Yu
Abstract:The system of Ditylenchus destructor reproduced monoxenically on Agrobacterium rhizogenes transformed root cultures between sweetpotato and carrot were established.The D.destructor's behavior were observed in vitro,and reproduction were investigated.The results showed:D.destructor can develop and reproduce in normal on hairy roots between sweetpotato and carrot,and the nematode was able to complete its life cycle;the production rate of D.destructor was 2.64 and 50.6 after 4 and 8 weeks on sweetpotato hairy roots,the production rate of D.destructor was 1.7 and 9.9 after 4 and 8 weeks on carrot hairy roots,the production on sweetpotato hairy roots was clearly more than on carrot hairy roots in the same time;using the system,the nematode's behavior could be clear observed through the microscope,it was very useful to study the plant-parasite interaction.Based on the results,The system of reproduction of D.destructor on sweetpotato hairy roots is feasible,and it was better than reproduction of D.destructor on carrot hairy roots.
Keywords:Ditylenchus destructor Sweetpotato hairy roots Carrot hairy roots Monoxenic culture
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