| 海参微卫星DNA的筛选与克隆的初步分析 |
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| 引用本文： | 杨磊,卢月娇,单雪,王秀利.海参微卫星DNA的筛选与克隆的初步分析[J].生物技术通报,2006(Z1):427-429. |
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| 作者姓名： | 杨磊 卢月娇 单雪 王秀利 |
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| 作者单位： | 大连水产学院生命科学与技术学院,大连,116023 |
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| 基金项目： | 大连水产学院校科研和教改项目 |
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| 摘 要： | 微卫星DNA(microsatelliteDNA)广泛存在于真核生物的基因组中。由于其具有突变频率快、多态性丰富、呈共显性遗传、通用性等特点,已成为近年来被广泛应用的分子遗传标记。对刺参基因组DNA的提取及其微卫星的筛选与克隆进行了初步分析。
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| 关 键 词： | 微卫星DNA PCR DNA提取 克隆 刺参 |
Preliminary Analysis of Cloning and Screening of Microsatellite DNA From Sea Cucumber(Stichopus japonicus) |
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| Yang Lei,Lu Yuejiao,Shan Xue,Wang Xiuli.Preliminary Analysis of Cloning and Screening of Microsatellite DNA From Sea Cucumber(Stichopus japonicus)[J].Biotechnology Bulletin,2006(Z1):427-429. |
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| Authors: | Yang Lei Lu Yuejiao Shan Xue Wang Xiuli |
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| Abstract: | Microsatellite DNA exists in eukaryotic genome widely. Nowadays, it has been applied abroadly as a molecular marker because of its rapid mutation frequency, abundant polymorphism, codominant inheritance, currency and so on. In this article, we made the preliminary analysis of extraction of genomic DNA, cloning and screening of microsatellite DNA from sea cucumber(Stichopus japonicus) |
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| Keywords: | Microsatellite DNA PCR DNA extraction Cloning Sea cucumber(Stichopus japonicus) |
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