| 新分离Microbacterium sp. XT11菌产黄原胶降解酶生产条件的优化研究 |
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| 引用本文： | 韩秋惠,李冲伟,王梅,金朝霞,李宪臻.新分离Microbacterium sp. XT11菌产黄原胶降解酶生产条件的优化研究[J].生物技术通报,2006(Z1):469-471. |
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| 作者姓名： | 韩秋惠 李冲伟 王梅 金朝霞 李宪臻 |
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| 作者单位： | 1. 大连轻工业学院生物与食品工程系,大连116034
2. 辽宁进出口商品检验检疫局理化检测中心,大连116001 |
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| 摘 要： | 新分离Microbacteriumsp.XT11菌能够合成黄原胶降解酶,将植物病原菌野油菜黄单孢菌分泌的毒素因子黄原胶分解,生成具有激发子和抗微生物活性的黄原胶寡糖。实验确认,黄原胶和酵母浸粉分别是XT11菌生产黄原胶降解酶的最适碳源和氮源,获得最高酶活力的最低碳源和氮源浓度均为0.3%。XT11菌生产黄原胶降解酶的最适条件为:培养温度28℃,培养基起始pH7.0,转速150r/min。
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| 关 键 词： | 黄原胶降解酶 黄原胶 发酵 生产条件 |
Production of Xanthan-Degrading Enzyme by Newly Isolated Microbacterium sp. XT11 |
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| Han Qiuhui,Li Chongwei,Wang Mei,Jin Zhaoxia,Li Xianzhen.Production of Xanthan-Degrading Enzyme by Newly Isolated Microbacterium sp. XT11[J].Biotechnology Bulletin,2006(Z1):469-471. |
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| Authors: | Han Qiuhui Li Chongwei Wang Mei Jin Zhaoxia Li Xianzhen |
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| Abstract: | The xanthan-degrading enzyme produced by newly isolated Microbacterium sp. XT11 could fragment xanthan which is the pathogen of phytobacterium Xanthomonas campestris pv. campestris. The xantho-oligosaccharides produced from xanthan have elicitor-active and antimicrobial activity. It has been shown that xanthan and yeast extract are respectively the optimum for the production of xanthan-degrading enzyme by XT11 strain, the minimum concentrations of which all are 0.3%. The optimal culture conditions for xanthan-degrading enzyme production by XT11 are that the XT11 is incubated at 28oC and 150 rpm in the culture at pH7.0. |
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| Keywords: | Microbacterium |
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