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两种核酸染料染色效果的比较研究
引用本文:蒋玲艳,王林果.两种核酸染料染色效果的比较研究[J].生物技术通报,2006(Z1):480-482.
作者姓名:蒋玲艳  王林果
作者单位:广西玉林师范学院化学与生物系,玉林,537000
摘    要:用前染和后染两种不同的染色方法,研究比较SYBRGreenI和溴化乙锭(EB)两种核酸染料对凝胶中DNA的染色效果和灵敏度,及SYBRGreenI取代EB用于常规凝胶中核酸染色的可能性。结果表明,用前染法染色SYBRGreenI对琼脂糖凝胶中的核酸染色效果与EB相当;用后染法染色前者要优于后者,可显示5ng以下的DNA条带,在完全相同的操作条件下,其染色DNA条带背景清晰,灵敏度较高。因此,无致突变性新型染料SYBRGreenI可替代强致突变性染料EB用于检测凝胶中DNA片段大小、含量等,从而减少由于使用EB带来的环境污染和人体健康危害。

关 键 词:溴化乙锭(EB)  SYBR  Green  I  核酸染色  效果比较  替代

A Comparative Study on the Staining Effect of Two Nucleic Acid Dyestuffs
Jiang Lingyan,Wang Linguo.A Comparative Study on the Staining Effect of Two Nucleic Acid Dyestuffs[J].Biotechnology Bulletin,2006(Z1):480-482.
Authors:Jiang Lingyan  Wang Linguo
Abstract:This study compare the staining effect and their sensitivity of SYBR Green I and Ethidium bromide (EB) to DNA in the Gels, by staining before electrophoresis and after electrophoresis. To evaluate the possible application of SYBR Green I in routine nucleic acid staining as a substitute for EB. The result showed that the staining effect of SYBR Green I and EB is uniform by staining before electrophoresis; but the sensitivity and definition of the former is better than the latter by staining after electrophoresis. SYBR Green I staining could detect less than 5ng per band of DNA. Therefore, SYBR Green I, which is a new and safe nucleic acid dyestuff without causing mutation, may substitute for EB as a general dyestuff to stain the DNA in the gel. Consequently, to decrease the pollution and harm due to using EB to stain.
Keywords:Ethidium Bromide(EB) SYBR Green I Nucleic acid dyestuff Staining effect Substitute
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